Anti-Cytokeratin 8 KRT8 Antibody Picoband™ (monoclonal, 3G9)

KRT8 antibody

Boster Bio Anti-Cytokeratin 8 KRT8 Antibody Picoband™ (monoclonal, 3G9) catalog # M01421-3. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M01421-3
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

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Product Name

Anti-Cytokeratin 8 KRT8 Antibody Picoband™ (monoclonal, 3G9)

View all KRT8 Antibodies

SKU/Catalog Number

M01421-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cytokeratin 8 KRT8 Antibody Picoband™ (monoclonal, 3G9) catalog # M01421-3. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytokeratin 8 KRT8 Antibody Picoband™ (monoclonal, 3G9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01421-3)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

3G9

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human Cytokeratin 8 recombinant protein (Position: D107-K325). Human Cytokeratin 8 shares 95.4% and 94.5% amino acid (aa) sequence identity with mouse and rat Cytokeratin 8, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M01421-3 is reactive to KRT8 in Human, Mouse, Rat

Applications

M01421-3 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Background of KRT8

Keratin, type II cytoskeletal 8, also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the KRT8 gene. This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunofluorescence, 2μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For KRT8 (Source: Uniprot.org, NCBI)

Uniprot ID

P05787

Gene ID

3856

Gene Name

KRT8

Full Name

Keratin, type II cytoskeletal 8

Weight

53.704kDa

Superfamily

intermediate filament family

Alternative Names

CARD2; CK8; CK-8; CYK8; Cytokeratin 8; Cytokeratin-8; EndoA; K2C8; K8; keratin 8; keratin, type II cytoskeletal 8; keratin-8; KO; KRT8; Type-II keratin Kb8 KRT8 CARD2, CK-8, CK8, CYK8, K2C8, K8, KO keratin 8 keratin, type II cytoskeletal 8|cytokeratin-8|keratin 8, type II|type-II keratin Kb8

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KRT8, check out the KRT8 Infographic

KRT8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KRT8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

M01421-3 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chen H,Kang J,Zhang F,Yan T,Fan W,He H,Huang F.SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions.Int J Biochem Cell Biol.2021 Feb 23:105962.doi:10.1016/j.biocel.2021.105962.Epub ahead of print.PMID:33636397.
Species: Rat
M01421-3 usage in article: APP:IF, SAMPLE:DPCS, DILUTION:1:100

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Total: $315

SKU:M01421-3

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M01421-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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