Human TIMP-4 ELISA Kit PicoKine™

Human TIMP-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TIMP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0524
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Product Name

Human TIMP-4 ELISA Kit PicoKine™

See all TIMP-4 products

SKU/Catalog Number

EK0524

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TIMP-4 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TIMP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TIMP-4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0524)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: sf21; Immunogen sequence: C30-P224

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0524 is reactive to TIMP4 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Application Guarantee

EK0524 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0524-CAP Anti-Human TIMP4 Pre-coated 96-well strip microplate 1
EK0524-ST Human TIMP4 Standard 2 vials, 10 ng/tube
EK0524-DA Human TIMP4 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078 156 312 625 1250 2500 5000
O.D.0.0060.1090.2320.5190.9981.3811.8092.166

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TIMP-4, Dilution ratio of 1:1, concentration in serum is around 1000pg/ml.

Some articles we found to cite concentrations of TIMP-4 in samples: 19114409 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TIMP-4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12358920561226011963
Standard deviation9.4738.87125.4110.9848.08155.07
CV (%)7.7%6.6%6.1%9%8%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TIMP-4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11231311191221234.433.6%
Sample 258961860559960210.491.7%
Sample 320562211202923432159126.555.8%
*number of samples for each test n=16.

Gene/Protein Information For TIMP4 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q99727

Gene ID

7079

Gene Name

TIMP4

Full Name

Metalloproteinase inhibitor 4

Weight

25.503kDa

Superfamily

protease inhibitor I35 (TIMP) family

Alternative Names

metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4 TIMP4 TIMP-4 TIMP metallopeptidase inhibitor 4 metalloproteinase inhibitor 4|tissue inhibitor of metalloproteinase 4|tissue inhibitor of metalloproteinases 4

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TIMP4, check out the TIMP4 Infographic

TIMP4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TIMP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0524 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expressions of MMP-12, TIMP-4, and Neutrophil Elastase in PBMCs and Exhaled Breath Condensate in Patients with COPD and Their Relationships with Disease Severity and Acute Exacerbations

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5 Customer Q&As for Human TIMP-4 ELISA Kit PicoKine™

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

C. Dhar

Verified customer

Asked: 2020-11-28

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-11-28

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

P. Wang

Verified customer

Asked: 2020-08-21

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-08-21

Question

Q: which procedure should I follow in order to thaw whole blood sample for TIMP-4 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TIMP-4 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-03-10

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of TIMP-4 in Human human milk? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-05-25

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TIMP-4 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-05-25

Question

Q: how to proceed with the analysis of ELISA data? I measured TIMP-4 level in human milk.

L. Maharaj

Verified customer

Asked: 2017-09-10

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-09-10

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