Product Info Summary
SKU: | PB10023 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ACADVL Antibody Picoband®
SKU/Catalog Number
PB10023
PB1076 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ACADVL Antibody Picoband® catalog # PB10023. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACADVL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10023)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ACADVL, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10023 is reactive to ACADVL in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
70390 MW
Background of ACADVL
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial (VLCAD) is an enzyme that in humans is encoded by the ACADVL gene. The protein encoded by this gene is targeted to the inner mitochondrial membrane, where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenaseis specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10023 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat liver extract (lane 1) and HELA whole cell (lane 2)
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ACADVL expression in rat liver extract (lane 1) and HELA whole cell lysates (lane 2). ACADVL at 66KD was detected using rabbit anti-ACADVL Antigen Affinity purified polyclonal antibody (Catalog # PB10023) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For ACADVL (Source: Uniprot.org, NCBI)
Gene Name
ACADVL
Full Name
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
Weight
70390 MW
Superfamily
acyl-CoA dehydrogenase family
Alternative Names
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial;VLCAD;1.3.8.9;ACADVL;VLCAD; ACADVL ACAD6, LCACD, VLCAD acyl-CoA dehydrogenase very long chain very long-chain specific acyl-CoA dehydrogenase, mitochondrial|acyl-Coenzyme A dehydrogenase, very long chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACADVL, check out the ACADVL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACADVL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACADVL Antibody Picoband® (PB10023)
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Customer Q&As
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4 Customer Q&As for Anti-ACADVL Antibody Picoband®
Question
Does anti-ACADVL antibody PB10023 work on canine WB with placenta?
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
Our lab technicians have not tested anti-ACADVL antibody PB10023 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ACADVL antibody PB10023 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-12
Question
Do you have a BSA free version of anti-ACADVL antibody PB10023 available?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ACADVL antibody PB10023 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-05
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-ACADVL antibody PB10023. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-22
Question
We are currently using anti-ACADVL antibody PB10023 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on zebrafish tissues as well?
R. Mangal
Verified customer
Asked: 2013-09-02
Answer
The anti-ACADVL antibody (PB10023) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-02