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- Table of Contents
ACP5 antibody (A03277-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of U251 cells using anti-Acid Phosphatase/ACP5 antibody (A03277-1).
Overlay histogram showing U251 cells stained with A03277-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Acid Phosphatase/ACP5 Antibody (A03277-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U251 cells stained with A03277-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Acid Phosphatase/ACP5 Antibody (A03277-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Acid phosphatase/ACP5 Antibody Picoband®
ACP5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ACP5 antibody was positively detected by WB in:
human HepG2 whole cell, human A431 whole cell, human Jurkat whole cell, human Hela whole cell, human K562 whole cell, human Raji whole cell, rat lung tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ACP5 antibody was positively detected by IHC in:
human spleen tissue, mouse lung tissue, human lung tissue, human renal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ACP5 antibody was positively detected by ICC/IF in:
MCF-7 cell
ACP5 antibody was positively detected by FC in:
U251 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Acid phosphatase/ACP5 Antibody Picoband®
View all ACP5 Antibodies
View all ACP5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Acid phosphatase/ACP5 recombinant protein (Position: N39-P325).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
39 kDa
Calculated Molecular Weight
36.6 kDa
Background of ACP5
Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5), is a glycosylated monomeric metalloprotein enzyme expressed in mammals. This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.
Cite This Product
Anti-Acid phosphatase/ACP5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03277-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
91%
High Homology
Rat
88%
Moderate Homology
Mouse
88%
Moderate Homology
Cow
87%
Moderate Homology
Sheep
87%
Moderate Homology
Rabbit
85%
Moderate Homology
Zebrafish
61%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ACP5 antibody
Immunohistochemistry (IHC) protocol for ACP5 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ACP5 antibody
Flow Cytometry (FC) protocol for ACP5 antibody
Publications
Application:
Species:
Extracellular vesicles derived from dental follicle stem cells regulate tooth eruption by inhibiting osteoclast differentiation
Published: 2024-12-20
Journal: Frontiers in Cell and Developmental Biology
Application: WB
Impact Factor: 4.60
Species: Mouse
PMID: 39834384
Salidroside promotes osteoblast proliferation and differentiation via the activation of AMPK to inhibit bone resorption of knee osteoarthritis mice
Published: 2022-09-05
Journal: TISSUE & CELL
Application: IHC
Impact Factor: 2.59
Species: Mouse
PMID: 36095933
JAK2-STAT3 signaling pathway is involved in rat periapical lesions induced by Enterococcus faecalis
Published: 2019-07-31
Journal: ORAL DISEASES
Application: IHC,WB
Impact Factor: 2.63
Species: Rat,Mouse
PMID: 31365165
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Product Promise
We promise Anti-Acid phosphatase/ACP5 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A03277-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026