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- Table of Contents
CASP12 antibody (PA2103) performs well in IHC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Anti-Caspase-12 antibody, PA2103, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
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Anti-Caspase-12/CASP12 Antibody Picoband®
CASP12 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | IHC, WB |
Tested Applications
CASP12 antibody was positively detected by WB in:
PANC Whole Cell, SMMC Whole Cell, A549 Whole Cell, HELA Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CASP12 antibody was positively detected by IHC in:
Human Mammary Cancer Tissue, Human Lung Cancer Tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Western blot
0.1-0.5μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Caspase-12/CASP12 Antibody Picoband®
View all CASP12 Antibodies
View all CASP12 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-12.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated Molecular Weight
38.9 kDa
Background of CASP12
Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. The CASP12 gene was mapped to chromosome 11q22.3. Csp12L attenuates the inflammatory and innate immune response to endotoxins and in doing so may constitute a risk factor for developing sepsis. The estrogen-mediated inhibition of CSP12L expression is a built-in mechanism that has evolved to protect females from infection by favoring a more robust inflammatory response to pathogens, perhaps notably in childbirth.
Cite This Product
Anti-Caspase-12/CASP12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2103)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
50%
Low homology
Monkey
43%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CASP12 antibody
Immunohistochemistry (IHC) protocol for CASP12 antibody
Publications
Application:
Species:
Taraxasterol attenuates zearalenone-induced kidney damage in mice by modulating oxidative stress and endoplasmic reticulum stress
Published: 2024-09-23
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB
Impact Factor: 6.20
Species: Mouse
PMID: 39317070
Tension induces intervertebral disc degeneration via endoplasmic reticulum stress-mediated autophagy
Published: 2019-08-07
Journal: BIOSCIENCE REPORTS
Application: IHC,WB
Impact Factor: 2.54
Species: Rat
PMID: 31285389
Bone marrow stromal cells improved functional recovery in spinal cord injury rats partly via the Toll‑like receptor‑4/nuclear factor‑κB signaling pathway
Published: 2018-10-31
Journal: Experimental and Therapeutic Medicine
Application: IHC
Impact Factor: 1.41
Species: Rat
PMID: 30651819
Isolation, characterization and in vitro anticancer activity of an aqueous galactomannan from the seed of Sesbania cannabina
Published: 2018-03-14
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: ICC
Impact Factor: 3.91
Species: Human
PMID: 29550424
ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction underlie apoptosis induced by resveratrol and arsenic trioxide in A549 cells
Published: 2016-01-06
Journal: CHEMICO-BIOLOGICAL INTERACTIONS
Application: WB
Impact Factor: 2.62
Species: Human
PMID: 26772155
Autophagy is involved in recombinant Newcastle disease virus (rL-RVG)-induced cell death of stomach adenocarcinoma cells in vitro
Published: 2015-06-09
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY
Application: WB
Impact Factor: 3.03
Species: Human
PMID: 26058483
Endoplasmic reticulum stress in diethylnitrosamine‑induced rat liver cancer
Published: 2013-10-29
Journal: Oncology Letters
Application: WB
Impact Factor: 0.24
Species: Rat
PMID: 24348814
Atmospheric Pressure Room Temperature Plasma Jets Facilitate Oxidative and Nitrative Stress and Lead to Endoplasmic Reticulum Stress Dependent Apoptosis in HepG2 Cells
Published: 2013-08-27
Journal: PLoS One
Application: WB
Impact Factor: 3.73
Species: Human
PMID: 24013954
Apoptotic effect of MG-132 on human tongue squamous cell carcinoma
Published: 2011-06-12
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 2.21
Species: Human
PMID: 21775096
Study of Histopathological and Molecular Changes of Rat Kidney under Simulated Weightlessness and Resistance Training Protective Effect
Published: 2011-05-23
Journal: PLoS One
Application: WB
Impact Factor: 4.41
Species: Rat
PMID: 21625440
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Product Promise
We promise Anti-Caspase-12/CASP12 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2103
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 25, 2026