Product Info Summary
| SKU: | M00752 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Acid sphingomyelinase (SMPD1) Mouse Monoclonal Antibody [Clone ID: OTI3H7]
SKU/Catalog Number
M00752
Size
100 µl
Description
Boster Bio SMPD1 mouse monoclonal antibody, clone OTI3H7 (formerly 3H7). Catalog# M00752. Tested in IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Acid sphingomyelinase (SMPD1) Mouse Monoclonal Antibody [Clone ID: OTI3H7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00752)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3H7
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 62-341 of human SMPD1 (NP_000534) produced in E.coli.
Reactive Species
M00752 is reactive to SMPD1 in Human
Calculated molecular weight
69.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00752 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:150
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMPD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMPD1.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Click image to see more details
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Click image to see more details
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Specific Publications For Anti-Acid sphingomyelinase (SMPD1) Mouse Monoclonal Antibody [Clone ID: OTI3H7] (M00752)
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