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- Table of Contents
ADK antibody (PB10031) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of ADK using anti-ADK antibody (PB10031).
ADK was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DDX1 Antibody (PB10031) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ADK was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DDX1 Antibody (PB10031) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-ADK Antibody Picoband®
ADK antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
ADK antibody was positively detected by WB in:
human placenta tissue, human PC-3 whole cell, human T47D whole cell, human U937 whole cell, human U251 whole cell, human HepG2 whole cell, rat liver tissue, rat kidney tissue, rat stomach tissue, rat PC-12 whole cell, mouse kidney tissue, mouse stomach tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ADK antibody was positively detected by IHC in:
human placenta tissue, mouse liver tissue, rat liver tissue, mouse kidney tissue, mouse liver tissue, human tosil tissue, human ovarian cancer tissue, human stomach cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ADK antibody was positively detected by ICC/IF in:
PC-3 cell
ADK antibody was positively detected by IF in:
human ovarian cancer tissue, human pancreas cancer tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-ADK Antibody Picoband®
View all ADK Antibodies
View all ADK Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ADK recombinant protein (Position: K165-T351). Human ADK shares 88.8% and 88.2% amino acid (aa) sequence identity with mouse and rat ADK, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated Molecular Weight
40.5 kDa
Background of ADK
This gene is an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-ADK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10031)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
97%
High Homology
Dog
93%
High Homology
Cow
93%
High Homology
Sheep
92%
High Homology
Mouse
89%
Moderate Homology
Rat
88%
Moderate Homology
Zebrafish
76%
Moderate Homology
Drosophila
51%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ADK antibody
Immunohistochemistry (IHC) protocol for ADK antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ADK antibody
Immunofluorescence (IF) protocol for ADK antibody
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Product Promise
We promise Anti-ADK Antibody Picoband® performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB10031
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026