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Product Info Summary
| SKU: | M02193-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-ADK Antibody (N-term)
SKU/Catalog Number
M02193-1
Size
50 µl
Description
ADK (adenosine kinase) catalyzes ATP-dependent phosphorylation of adenosine to AMP, acting as a key regulator of cellular adenosine availability and purine salvage. Assay context: human-reactive antibody tested for Western blot and paraffin IHC (per provided product description), supporting tissue-localization of adenosine-metabolism capacity in disease models where extracellular adenosine tone is being studied (interpretation context dependent; putative).
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ADK Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02193-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1103CT3.4.3
Isotype
IgG1,k
Immunogen
This ADK antibody is generated from a mouse immunized with a recombinant protein between 1-345 amino acids from human ADK.
Reactive Species
M02193-1 is reactive to ADK in Human
Calculated molecular weight
40.5 kDa
Background of ADK
ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02193-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-ADK Antibody (N-term) at 1:4000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: HL-60 whole cell lysate
Lane 3: Human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 45 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M02193-1 staining ADK in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-ADK Antibody (N-term) (M02193-1)
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