Product Info Summary
SKU: | M02193-1 |
---|---|
Size: | 50 µl |
Reactive Species: | Human |
Host: | Mouse |
Application: | IHC-P, WB |
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Product info
Product Name
Anti-ADK Antibody (N-term)
SKU/Catalog Number
M02193-1
Size
50 µl
Description
Boster Bio Anti-ADK Antibody (N-term) (Catalog # M02193-1). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ADK Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02193-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1103CT3.4.3
Isotype
IgG1,k
Immunogen
This ADK antibody is generated from a mouse immunized with a recombinant protein between 1-345 amino acids from human ADK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02193-1 is reactive to ADK in Human
Applications
M02193-1 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
40.545kDa
Background of ADK
ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
IHC-P: 1:25
Validation Images & Assay Conditions
Click image to see more details
All lanes : Anti-ADK Antibody (N-term) at 1:4000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: HL-60 whole cell lysate
Lane 3: Human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 45 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
M02193-1 staining ADK in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For ADK (Source: Uniprot.org, NCBI)
Gene Name
ADK
Full Name
Adenosine kinase
Weight
40.545kDa
Superfamily
carbohydrate kinase PfkB family
Alternative Names
adenosine kinase; ADK; AK; AKAdenosine 5'-phosphotransferase; EC 2.7.1; EC 2.7.1.20 ADK AK adenosine kinase adenosine kinase|adenosine 5-phosphotransferase|testicular tissue protein Li 14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADK, check out the ADK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADK Antibody (N-term) (M02193-1)
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