Product Info Summary
SKU: | PB9470 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Angiotensin II Type 1 Receptor/AGTR1 Antibody Picoband™
SKU/Catalog Number
PB9470
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Angiotensin II Type 1 Receptor/AGTR1 Antibody Picoband™ catalog # PB9470. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiotensin II Type 1 Receptor/AGTR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9470)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human AGTR1, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9470 is reactive to AGTR1 in Human, Monkey, Mouse, Rat
Applications
PB9470 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
41.061kDa
Background of AGTR-1
AGTR1 is known as Angiotensin II Type 1 Receptor. Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. And it acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. Moreover, this gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AGTR1 using anti-AGTR1 antibody (PB9470).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: monkey COS-7 whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: rat liver tissue lysates,
Lane 9: rat kidney tissue lysates,
Lane 10: rat RH35 whole cell lysates,
Lane 11: mouse brain tissue lysates,
Lane 12: mouse liver tissue lysates,
Lane 13: mouse kidney tissue lysates,
Lane 14: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGTR1 antigen affinity purified polyclonal antibody (Catalog # PB9470) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGTR1 at approximately 50 kDa. The expected band size for AGTR1 is at 41 kDa.
Click image to see more details
Figure 2. IHC analysis of AGTR1 using anti-AGTR1 antibody (PB9470).
AGTR1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AGTR1 Antibody (PB9470) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For AGTR1 (Source: Uniprot.org, NCBI)
Gene Name
AGTR1
Full Name
Type-1 angiotensin II receptor
Weight
41.061kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
AG2S; AGTR1; AGTR-1; AGTR1AAngiotensin II type-1 receptor; Angiostensin Receptor; angiotensin II receptor, type 1; angiotensin receptor 1; angiotensin receptor 1B; AT1; AT1AR; AT1AT1AR; AT1B; AT1BR; AT1R; AT2R1; AT2R1A; AT2R1AGTR1B; AT2R1BAT1BR; HAT1R; type-1 angiotensin II receptor; type-1B angiotensin II receptor AGTR1 AG2SB, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, HAT1R, AGTR1 angiotensin II receptor type 1 type-1 angiotensin II receptor|type-1B angiotensin II receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AGTR1, check out the AGTR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AGTR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Angiotensin II Type 1 Receptor/AGTR1 Antibody Picoband™ (PB9470)
Hello CJ!
PB9470 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Angiotensin II induces apoptosis of human pulmonary microvascular endothelial cells in acute aortic dissection complicated with lung injury patients through modulating the expression of monocyte chemoattractant protein-1
Effects of angiotensin II receptor antagonist, Losartan on the apoptosis, proliferation and migration of the human pancreatic stellate cells
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Customer Q&As
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6 Customer Q&As for Anti-Angiotensin II Type 1 Receptor/AGTR1 Antibody Picoband™
Question
I see that the anti-Angiotensin II Type 1 Receptor/AGTR1 antibody PB9470 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-05-05
Question
I am interested in your PB9470 AGTR1 antibody, may I know if the antibody could be used with mouse and rat samples by WB and IHC? Please let me know if you have related information.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
We failed to get positive staining for IHC with mouse and rat samples. WB test result on rat samples was not good.
Boster Scientific Support
Answered: 2019-12-06
Question
My question regarding product PB9470, anti-Angiotensin II Type 1 Receptor/AGTR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Krishna
Verified customer
Asked: 2017-10-17
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9470 anti-Angiotensin II Type 1 Receptor/AGTR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-17
Question
Do you have a BSA free version of anti-Angiotensin II Type 1 Receptor/AGTR1 antibody PB9470 available?
S. Brown
Verified customer
Asked: 2017-08-17
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Angiotensin II Type 1 Receptor/AGTR1 antibody PB9470 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-17
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Angiotensin II Type 1 Receptor/AGTR1 antibody PB9470. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-16
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-16
Question
We are currently using anti-Angiotensin II Type 1 Receptor/AGTR1 antibody PB9470 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?
L. Gonzalez
Verified customer
Asked: 2016-06-27
Answer
The anti-Angiotensin II Type 1 Receptor/AGTR1 antibody (PB9470) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-27