Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband®

Product Name

Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband®

View all PIAS1 Antibodies

SKU/Catalog Number

PA2252
BA3491 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody catalog # PA2252. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2252)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS1, identical to the related mouse and rat sequences.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2252 is reactive to PIAS1 in Human, Mouse, Rat

Observed Molecular Weight

100 kDa

Calculated molecular weight

71.8 kDa

Background of PIAS1

E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. On the other hand, PIAS1 also can function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2252 is guaranteed for IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat

Positive Control

WB: Rat Testis Tissue, Mouse Testis Tissue, HELA Whole Cell, JURKAT Whole Cell, MCF-7 Whole Cell, SKOV Whole Cell
IHC: Rat Testis Tissue, Rat Brain Tissue, Rat Brain Tissue, Human Placenta Tissue
ICC: A549 Cell

Validation Images & Assay Conditions

See full target information PIAS1

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