Anti-AHSP/Eraf Antibody

ERAF antibody

Boster Bio Anti-AHSP/Eraf Antibody (Catalog # A05948). Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A05948
Size: 100uL
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC, WB

Product Name

Anti-AHSP/Eraf Antibody

View all ERAF Antibodies

SKU/Catalog Number

A05948

Size

100uL

Form

Lyophilized

Description

Boster Bio Anti-AHSP/Eraf Antibody (Catalog # A05948). Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-AHSP/Eraf Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05948)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

Anti-AHSP Antibody was produced from whole rabbit serum prepared by repeated immunizations with the full length human AHSP protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05948 is reactive to AHSP in Human, Mouse

Applications

A05948 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

11.84kDa

Background of ERAF

AHSP Antibody detects Alpha hemoglobin stabilizing protein (AHSP). AHSP acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. AHSP binds free a-globin to promote its folding and inhibit its ability to produce damaging reactive oxygen species. Reduced AHSP levels correlate with increased severity of b-thalassemia in some human cohorts.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: 1:8000
WB: 1:500-1:1000

Validation Images & Assay Conditions

Gene/Protein Information For AHSP (Source: Uniprot.org, NCBI)

Gene Name

AHSP

Full Name

Alpha-hemoglobin-stabilizing protein

Weight

11.84kDa

Superfamily

AHSP family

Alternative Names

alpha hemoglobin stabilizing protein; EDRFalpha hemoglobin stabilising protein; ERAFalpha-hemoglobin-stabilizing protein; erythroid associated factor; erythroid differentiation associated factor; Erythroid differentiation-related factor; Erythroid-associated factor AHSP EDRF, ERAF alpha hemoglobin stabilizing protein alpha-hemoglobin-stabilizing protein|alpha hemoglobin stabilising protein|erythroid differentiation associated factor|erythroid differentiation-related factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AHSP, check out the AHSP Infographic

AHSP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AHSP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-AHSP/Eraf Antibody

Question

What kind of buffer and how much to use in the reconstitution of A05948?

Verified customer

Asked: 2020-11-13

Answer

The Anti-AHSP/Eraf Antibody (A05948) may be restored with deionized water (or equivalent) to 100ul volume.

Boster Scientific Support

Answered: 2020-11-13

Question

We have the sample size of A05948. How much buffer should we use? Can you tell me the final concentration after the reconstitution?

Verified customer

Asked: 2020-11-13

Answer

The sample size of the Anti-AHSP/Eraf Antibody (A05948) may be reconstituted in deionized water to a final volume of 25ul. This will allow to use antibody at dilutions listed on the datasheet. Alternatively, our company also proposes that this vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions.

Boster Scientific Support

Answered: 2020-11-13

Question

Does A05948 require the antigen retrieval? If yes, what’s the buffer pH?

Verified customer

Asked: 2020-11-13

Answer

For the Anti-AHSP/Eraf Antibody (A05948), please use Sodium Citrate pH 6.5 if that’s what you are doing. Our data was generated on acetic acid-zinc-formalin fixed samples and TRIS-EDTA pH 9.0 was our retrieval buffer.

Boster Scientific Support

Answered: 2020-11-16

Question

We are currently using anti-AHSP/Eraf antibody A05948 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?

B. Wu

Verified customer

Asked: 2020-02-05

Answer

The anti-AHSP/Eraf antibody (A05948) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-05

Size

Total: $599

SKU:A05948

Backordered.

Lead time for this item is typically 4-6 days

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A05948
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$599.00

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