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SKU:MA1090
Pack Size:100μg/vial
Validated Species:Human
Conjugate:No
Clonality:Monoclonal
Clone Number:SB-SP1
Host:Mouse
Application:WB
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Overview

Product Name Anti-Spectrin(Alpha And Beta) Antibody (Monoclonal, SB-SP1)
SKU/Catalog Number MA1090
Description Mouse IgG monoclonal antibody for Spectrin(alpha and beta), spectrin, alpha, erythrocytic 1 (elliptocytosis 2); spectrin, beta, erythrocytic (SPTA1; SPTB ) detection. Tested with WB in Human. No cross reactivity with other proteins.
Cite This Product Anti-Spectrin(Alpha And Beta) Antibody (Monoclonal, SB-SP1) SB-SP1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1090)
Replacement Item This antibody may replace the following items: sc-15371|sc-271130|sc-28271|sc-374308|sc-374309|sc-376859|sc-377437|sc-53900|sc-53901|sc-58851|sc-73053|sc-7464|sc-7466|sc-7467 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human erythrocyte spectrin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number SB-SP1
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Sptb
Protein Name Spectrin alpha chain, erythrocytic 1; Spectrin beta chain, erythrocytic
Molecular Weight 246329 MW
Protein Function SPTA1: Spectrin is the major constituent of the cytoskeletalnetwork underlying the erythrocyte plasma membrane. It associateswith band 4.1 and actin to form the cytoskeletal superstructure ofthe erythrocyte plasma membrane.

SPTB: Spectrin is the major constituent of the cytoskeletalnetwork underlying the erythrocyte plasma membrane. It associateswith band 4.1 and actin to form the cytoskeletal superstructure ofthe erythrocyte plasma membrane.
Sequence Similarities Belongs to the spectrin family.
Subcellular Localization SPTA1: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.
Spta1: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.
SPTB: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.
Sptb: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.
Uniprot ID Q6XDA0
Alternative Names Erythroid spectrin beta ;Sptb ;LOC314251 ;
Research Areas tags & cell markers|subcellular markers|plasma membrane|signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin crosslinking|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Spectrin alpha chain, erythrocytic 1; Spectrin beta chain, erythrocytic

Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2. Spectrin mutations cause spinocerebellar ataxia type 5.
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is erythrocyte antibody