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Product Info Summary
| SKU: | M02303 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-AKAP12 Mouse Monoclonal Antibody [Clone ID: OTI1B6]
SKU/Catalog Number
M02303
Size
100 µl
Description
Boster Bio AKAP12 mouse monoclonal antibody,clone OTI1B6. Catalog# M02303. Tested in IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-AKAP12 Mouse Monoclonal Antibody [Clone ID: OTI1B6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02303)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1B6
Isotype
IgG2b
Immunogen
Human recombinant protein fragment corresponding to amino acids 266-557 of human AKAP12 (NP_005091) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02303 is reactive to AKAP12 in Human
Reconstitution
Observed Molecular Weight
191 kDa
Calculated molecular weight
191.482kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02303 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of AKAP12 using anti-AKAP12 antibody (M02303).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AKAP12 antigen affinity purified monoclonal antibody (Catalog # M02303) at at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for AKAP12 at approximately 191 kDa. The expected band size for AKAP12 is at 191 kDa.
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Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-AKAP12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-AKAP12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-AKAP12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human adult heart tissue within the normal limits using anti-AKAP12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Protein Target Info & Infographic
Gene/Protein Information For AKAP12 (Source: Uniprot.org, NCBI)
Gene Name
AKAP12
Full Name
A-kinase anchor protein 12
Weight
191.482kDa
Alternative Names
A kinase (PRKA) anchor protein (gravin) 12; A kinase (PRKA) anchor protein 12; AKAP 250; AKAP250250kDa; DKFZp686M0430; DKFZp686O0331; FLJ20945; FLJ97621; Gravin; kinase scaffold protein gravin; myasthenia gravis autoantigen gravin; Myasthenia gravis autoantigen AKAP12 AKAP250, SSeCKS A-kinase anchoring protein 12 A-kinase anchor protein 12|A kinase (PRKA) anchor protein 12|A-kinase anchor protein, 250kDa|AKAP 250|Src-Suppressed C Kinase Substrate|kinase scaffold protein gravin|myasthenia gravis auto gravin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKAP12, check out the AKAP12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKAP12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AKAP12 Mouse Monoclonal Antibody [Clone ID: OTI1B6] (M02303)
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