Anti-AKT (PH domain) AKT1 Rabbit Monoclonal Antibody, Clone#RM316

Akt1 antibody

Boster Bio Anti-AKT (PH domain) AKT1 Rabbit Monoclonal Antibody, Clone#RM316 (Catalog # M00024-4). Tested in IHC, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: M00024-4
Size: 100uL
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-AKT (PH domain) AKT1 Rabbit Monoclonal Antibody, Clone#RM316

View all Akt1 Antibodies

SKU/Catalog Number

M00024-4

Size

100uL

Form

Liquid

Description

Boster Bio Anti-AKT (PH domain) AKT1 Rabbit Monoclonal Antibody, Clone#RM316 (Catalog # M00024-4). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AKT (PH domain) AKT1 Rabbit Monoclonal Antibody, Clone#RM316 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00024-4)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM316

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to the PH domain of human AKT

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to human AKT (PH domain). It may also react to mouse or rat AKT, as predicted by immunogen homology.

Reactive Species

M00024-4 is reactive to AKT1 in Human

Applications

M00024-4 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

55.686kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:200-1:500 dilution
WB: 1:5000-1:10000 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For AKT1 (Source: Uniprot.org, NCBI)

Gene Name

AKT1

Full Name

RAC-alpha serine/threonine-protein kinase

Weight

55.686kDa

Superfamily

protein kinase superfamily

Alternative Names

AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AKT1, check out the AKT1 Infographic

AKT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AKT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00024-4 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse
M00024-4 usage in article: APP:WB, SAMPLE:SNU-16 CELL, DILUTION:NA

A small interfering RNA targeting osteopontin as gastric cancer therapeutics

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16 Customer Q&As for Anti-AKT (PH domain) AKT1 Rabbit Monoclonal Antibody, Clone#RM316

Question

My lab would like using your anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 for negative regulation of notch signaling pathway studies. Has this antibody been tested with western blotting on 293 cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

Thanks for your inquiry. This M00024-4 anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 is tested on 293 cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-07

Question

See attached the WB image, lot number and protocol we used for foreskin using anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 M00024-4. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-31

Question

I was wanting to use your anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 for IHC for human foreskin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human foreskin identification?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

You can see on the product datasheet, M00024-4 anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human foreskin in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-20

Question

My question regarding product M00024-4, anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00024-4 anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-10

Question

Do you have a BSA free version of anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 M00024-4 available?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 M00024-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-22

Question

you antibody to test anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 M00024-4 on human foreskin for research purposes, then I may be interested in using anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 M00024-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

The products we sell, including anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 M00024-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-01

Question

Our team were content with the WB result of your anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

From what I have seen in literature, left adrenal gland does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-24

Question

We have been able to see staining in human cervix carcinoma. What should we do? Is anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2019-05-07

Answer

According to literature cervix carcinoma does express AKT1. According to Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-05-07

Question

Is this M00024-4 anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 reactive to the isotypes of AKT1?

Verified Customer

Verified customer

Asked: 2018-09-05

Answer

The immunogen of M00024-4 anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 is A peptide corresponding to the PH domain of human AKT. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-09-05

Question

Would anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 M00024-4 work for IHC with foreskin?

Verified Customer

Verified customer

Asked: 2018-07-20

Answer

According to the expression profile of foreskin, AKT1 is highly expressed in foreskin. So, it is likely that anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 M00024-4 will work for IHC with foreskin.

Boster Scientific Support

Answered: 2018-07-20

Question

Would M00024-4 anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-06-29

Answer

As indicated on the product datasheet, M00024-4 anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-06-29

Question

We purchased anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2018-02-02

Answer

I looked at the website and datasheets of our anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2018-02-02

Question

I see that the anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 M00024-4 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-12-20

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-12-20

Question

We are currently using anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 M00024-4 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?

M. Jones

Verified customer

Asked: 2017-01-06

Answer

The anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 (M00024-4) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-01-06

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for foreskin using anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 M00024-4. Let me know if you need anything else.

C. Gonzalez

Verified customer

Asked: 2016-05-11

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-05-11

Question

Is a blocking peptide available for product anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 (M00024-4)?

G. Baker

Verified customer

Asked: 2015-07-16

Answer

We do provide the blocking peptide for product anti-AKT (PH domain) Rabbit Monoclonal antibody, Clone#RM316 (M00024-4). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-07-16

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Lead time for this item is typically 1-2 weeks

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M00024-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$431.00

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