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- Table of Contents
IKBKB antibody (PB9221) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of 293T cells using anti-IKK beta antibody (PB9221).
Overlay histogram showing 293T cells stained with PB9221 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK beta Antibody (PB9221,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing 293T cells stained with PB9221 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK beta Antibody (PB9221,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot or IHC for IKBKB? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-IKK beta/IKBKB Antibody Picoband®
IKBKB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
IKBKB antibody was positively detected by WB in:
HEPG2 Whole Cell, COLO320 Whole Cell, M231 Whole Cell, HT1080 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
IKBKB antibody was positively detected by IHC in:
Human Lung Cancer Tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
IKBKB antibody was positively detected by ICC/IF in:
A431 cell
IKBKB antibody was positively detected by FC in:
293T cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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IHC
IF
Human
Tested
Recommend
Monkey
Predicted
Predicted
Rabbit
Predicted
Predicted
Dog
Predicted
Predicted
Mouse
Predicted
Predicted
Bovine
Predicted
Predicted
Product Information
Product Name
Anti-IKK beta/IKBKB Antibody Picoband®
View all IKBKB Antibodies
View all IKBKB Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IKK beta recombinant protein (Position: E398-S756). Human IKK beta shares 91% and 90% amino acid (aa) sequences identity with mouse and rat IKK beta, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated Molecular Weight
86.6 kDa
Background of IKBKB
IKBKB (Inhibitor of Kappa Light Chain Gene Enhancer in B Cells, Kinase of, Beta), also known as IKKB or NFKBIKB, is a protein that in humans is encoded by the IKBKB gene. This gene is mapped to chromosome 8p12-p11 by FISH. It is found that mutations in IKK2 had a more pronounced effect upon NFKB activation than did comparable mutations in IKK1. The activity of various antiinflammatory agents on the IKK complex has been texted, it has been found that aspirin and sodium salicylate specifically inhibit IKK-beta activity in vitro and in vivo by binding to IKK-beta to reduce ATP binding.
Cite This Product
Anti-IKK beta/IKBKB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9221)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
94%
High Homology
Dog
93%
High Homology
Mouse
91%
High Homology
Cow
91%
High Homology
Rat
89%
Moderate Homology
Sheep
88%
Moderate Homology
Zebrafish
50%
Low homology
Drosophila
16%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for IKBKB antibody
Immunohistochemistry (IHC) protocol for IKBKB antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for IKBKB antibody
Flow Cytometry (FC) protocol for IKBKB antibody
Publications
Application:
Species:
Baicalin ameliorates neuroinflammation by targeting TLR4/MD2 complex on microglia via PI3K/AKT/NF-κB signaling pathway
Published: 2025-01-09
Journal: NEUROPHARMACOLOGY
Application: WB
Impact Factor: 4.60
Species: Mouse
PMID: 39798687
Review
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Product Promise
We promise Anti-IKK beta/IKBKB Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
PB9221
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 28, 2026