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Product Info Summary
| SKU: | A00024T450-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-AKT (phospho-T450) AKT1 Antibody
SKU/Catalog Number
A00024T450-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-AKT (phospho-T450) AKT1 Antibody catalog # A00024T450-1. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AKT (phospho-T450) AKT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00024T450-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 450.
Reactive Species
A00024T450-1 is reactive to AKT1 in Human, Mouse, Rat
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00024T450-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of AKT (phospho-T450) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with EGF(0.1ng/ml,5min) whole cell lysate Lane3:L02 treated with EGF(0.1ng/ml,10min) whole cell lysate Lane4:L02 treated with EGF(0.1ng/ml,15min) whole cell lysate
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Immunohistochemistry (IHC) analyzes of p-AKT (T450) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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Immunofluorescence analysis of NIH-3T3 cells using AKT (phospho-T450) pAb at dilution of 1:200 (40x lens).
Specific Publications For Anti-AKT (phospho-T450) AKT1 Antibody (A00024T450-1)
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