Anti-AKT1 Monoclonal Antibody

Akt1 antibody

Boster Bio Anti-AKT1 Monoclonal Antibody catalog # M00024-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: M00024-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

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Product Name

Anti-AKT1 Monoclonal Antibody

View all Akt1 Antibodies

SKU/Catalog Number

M00024-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-AKT1 Monoclonal Antibody catalog # M00024-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AKT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00024-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EBG-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00024-1 is reactive to AKT1 in Human, Mouse, Rat

Applications

M00024-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

55.686kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For AKT1 (Source: Uniprot.org, NCBI)

Gene Name

AKT1

Full Name

RAC-alpha serine/threonine-protein kinase

Weight

55.686kDa

Superfamily

protein kinase superfamily

Alternative Names

AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AKT1, check out the AKT1 Infographic

AKT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AKT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00024-1 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The calcimimetic R-568 attenuates subarachnoid hemorrhage-induced vasospasm through PI3K/Akt/eNOS signaling pathway in the rat model

Güleç İ, Şengelen A,Karagöz-Güzey F,Önay-Uçar E,Eren B,Vahabova G,Karacan M,Bilgen Özcan T.The calcimimetic R-568 attenuates subarachnoid hemorrhage-induced vasospasm through PI3K/Akt/eNOS signaling pathway in the rat model.Brain Res.2021 Apr 27:147508.doi:10.1016/j. brainres.2021. 147508.Epub ahead of print.PMID:33930376.
Species: Rat
M00024-1 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION: 1:1000

Qu K,Cha H, Ru Y,Que H,Xing M.Buxuhuayu Decoction Accelerates Angiogenesis by Activating the PI3K-Akt-eNOS Signalling Pathway in a Streptozotocin-Induced Diabetic Ulcer Rat Model.J Ethnopharmacol.2021 Feb 10:113824.doi:10.1016/j.jep.2021.113824.Epub ahead
Species: Rat
M00024-1 usage in article: APP:WB, SAMPLE:WOUND TISSUE, DILUTION:1:1000

A small interfering RNA targeting osteopontin as gastric cancer therapeutics

Short hairpin RNA targeting AKT1 and PI3K/p85 suppresses the proliferation and self-renewal of lung cancer stem cells

Protective Effects of Sodium (?)-5-Bromo-2-(%u03B1-Hydroxypentyl) Benzoate in a Rodent Model of Global Cerebral Ischemia

Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways

Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways

Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice

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16 Customer Q&As for Anti-AKT1 Monoclonal Antibody

Question

See below the WB image, lot number and protocol we used for adrenal gland using anti-AKT1 Monoclonal antibody M00024-1. Please let me know if you require anything else.

A. Krishna

Verified customer

Asked: 2020-03-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-10

Question

My boss were content with the WB result of your anti-AKT1 Monoclonal antibody. However we have observed positive staining in cervix carcinoma cytoplasm. using this antibody. Is that expected? Could you tell me where is AKT1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

From literature, cervix carcinoma does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-02-14

Question

Is this M00024-1 anti-AKT1 Monoclonal antibody reactive to the isotypes of AKT1?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

The immunogen of M00024-1 anti-AKT1 Monoclonal antibody is A synthesized peptide derived from human AKT1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-23

Question

Is a blocking peptide available for product anti-AKT1 Monoclonal antibody (M00024-1)?

A. Moore

Verified customer

Asked: 2019-09-12

Answer

We do provide the blocking peptide for product anti-AKT1 Monoclonal antibody (M00024-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-12

Question

We have observed staining in human cervix carcinoma. What should we do? Is anti-AKT1 Monoclonal antibody supposed to stain cervix carcinoma positively?

G. Bhatt

Verified customer

Asked: 2019-06-04

Answer

According to literature cervix carcinoma does express AKT1. According to Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-04

Question

I see that the anti-AKT1 Monoclonal antibody M00024-1 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-07-05

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-05

Question

Our lab used your anti-AKT1 Monoclonal antibody for WB on muscle ovary a few years ago. I am using mouse, and I plan to use the antibody for IHC next. Our lab want to know about examining muscle ovary as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-06-20

Answer

I viewed the website and datasheets of our anti-AKT1 Monoclonal antibody and it seems that M00024-1 has been tested on mouse in both WB and IHC. Thus M00024-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-06-20

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adrenal gland using anti-AKT1 Monoclonal antibody M00024-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-05-04

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-04

Question

My question regarding product M00024-1, anti-AKT1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Jones

Verified customer

Asked: 2017-07-03

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00024-1 anti-AKT1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-07-03

Question

Would anti-AKT1 Monoclonal antibody M00024-1 work for ICC with adrenal gland?

N. Krishna

Verified customer

Asked: 2016-11-21

Answer

According to the expression profile of adrenal gland, AKT1 is highly expressed in adrenal gland. So, it is likely that anti-AKT1 Monoclonal antibody M00024-1 will work for ICC with adrenal gland.

Boster Scientific Support

Answered: 2016-11-21

Question

I would like to test anti-AKT1 Monoclonal antibody M00024-1 on mouse adrenal gland for research purposes, then I may be interested in using anti-AKT1 Monoclonal antibody M00024-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Rodriguez

Verified customer

Asked: 2016-09-28

Answer

The products we sell, including anti-AKT1 Monoclonal antibody M00024-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-09-28

Question

Do you have a BSA free version of anti-AKT1 Monoclonal antibody M00024-1 available?

S. Li

Verified customer

Asked: 2015-05-15

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AKT1 Monoclonal antibody M00024-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-05-15

Question

Would M00024-1 anti-AKT1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Evans

Verified customer

Asked: 2015-04-10

Answer

It shows on the product datasheet, M00024-1 anti-AKT1 Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-04-10

Question

We are interested in using your anti-AKT1 Monoclonal antibody for cellular response to dna damage stimulus studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

G. Jones

Verified customer

Asked: 2015-04-03

Answer

We appreciate your inquiry. This M00024-1 anti-AKT1 Monoclonal antibody is validated on hela cells. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-04-03

Question

We are currently using anti-AKT1 Monoclonal antibody M00024-1 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

R. Singh

Verified customer

Asked: 2013-10-01

Answer

The anti-AKT1 Monoclonal antibody (M00024-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-10-01

Question

I was wanting to use your anti-AKT1 Monoclonal antibody for ICC for mouse adrenal gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse adrenal gland identification?

K. Moore

Verified customer

Asked: 2013-09-13

Answer

As indicated on the product datasheet, M00024-1 anti-AKT1 Monoclonal antibody has been validated for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse adrenal gland in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-09-13

Size

Total: $370

SKU:M00024-1

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Lead time for this item is typically 3-4 weeks

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M00024-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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