Product Info Summary
SKU: | M00024-3 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Akt1 Rabbit Monoclonal Antibody, Clone#RM252
SKU/Catalog Number
M00024-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Akt1 Rabbit Monoclonal Antibody, Clone#RM252 (Catalog # M00024-3). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Akt1 Rabbit Monoclonal Antibody, Clone#RM252 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00024-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM252
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to the C-terminus of human Akt1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Akt1. This antibody may also react to bovine, mouse or rat Akt1, as predicted by immunogen homology
Reactive Species
M00024-3 is reactive to AKT1 in Human
Applications
M00024-3 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
55.686kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western Blotting result
Western Blot of 293 cell lysate, using Anti-Akt1 RM252 at a 1:1000 dilution, showed Akt1 (~56 kDa) expression in 293 cells.
Click image to see more details
Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Akt1 RM252 at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For AKT1 (Source: Uniprot.org, NCBI)
Gene Name
AKT1
Full Name
RAC-alpha serine/threonine-protein kinase
Weight
55.686kDa
Superfamily
protein kinase superfamily
Alternative Names
AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKT1, check out the AKT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Akt1 Rabbit Monoclonal Antibody, Clone#RM252 (M00024-3)
Hello CJ!
M00024-3 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Investigating function of long noncoding RNA of HOTAIRM1 in progression of SKOV3 ovarian cancer cells
A small interfering RNA targeting osteopontin as gastric cancer therapeutics
Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Akt1 Rabbit Monoclonal Antibody, Clone#RM252
Question
I would like to test anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 M00024-3 on human muscle ovary for research purposes, then I may be interested in using anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 M00024-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Zhao
Verified customer
Asked: 2020-01-13
Answer
The products we sell, including anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 M00024-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-13
Question
I was wanting to use your anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 for IHC for human muscle ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human muscle ovary identification?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
As indicated on the product datasheet, M00024-3 anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human muscle ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-14
Question
I see that the anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 M00024-3 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-30
Question
Our lab were well pleased with the WB result of your anti-Akt1 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
From what I have seen in literature, liver does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-12
Question
See attached the WB image, lot number and protocol we used for muscle ovary using anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 M00024-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-12
Question
Is this M00024-3 anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 reactive to the isotypes of AKT1?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The immunogen of M00024-3 anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 is A synthetic peptide corresponding to the C-terminus of human Akt1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-21
Question
Is a blocking peptide available for product anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 (M00024-3)?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
We do provide the blocking peptide for product anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 (M00024-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-06
Question
My question regards using your anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 for intracellular signal transduction studies. Has this antibody been tested with western blotting on 293 cells? We would like to see some validation images before ordering.
T. Parker
Verified customer
Asked: 2019-05-01
Answer
I appreciate your inquiry. This M00024-3 anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 is tested on 293 cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-01
Question
We have tried in the past anti-Akt1 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-03-14
Answer
I took a look at the website and datasheets of our anti-Akt1 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-03-14
Question
My question regarding product M00024-3, anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00024-3 anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-28
Question
We are currently using anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 M00024-3 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
The anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 (M00024-3) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-18
Question
Do you have a BSA free version of anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 M00024-3 available?
G. Bhatt
Verified customer
Asked: 2018-10-30
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 M00024-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-30
Question
We have observed staining in human muscle ovary. Are there any suggestions? Is anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 supposed to stain muscle ovary positively?
Verified Customer
Verified customer
Asked: 2017-11-22
Answer
According to literature muscle ovary does express AKT1. According to Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-11-22
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle ovary using anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 M00024-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-06
Question
Would anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 M00024-3 work for IHC with muscle ovary?
S. Zhang
Verified customer
Asked: 2016-06-15
Answer
According to the expression profile of muscle ovary, AKT1 is highly expressed in muscle ovary. So, it is likely that anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 M00024-3 will work for IHC with muscle ovary.
Boster Scientific Support
Answered: 2016-06-15
Question
Would M00024-3 anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Anderson
Verified customer
Asked: 2013-05-21
Answer
As indicated on the product datasheet, M00024-3 anti-Akt1 Rabbit Monoclonal antibody, Clone#RM252 as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-05-21