Click image to see more details
Product Info Summary
| SKU: | M00725-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-AKT2 Antibody Picoband® (monoclonal, 10C6)
SKU/Catalog Number
M00725-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AKT2 Antibody Picoband® (monoclonal, 10C6) catalog # M00725-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AKT2 Antibody Picoband® (monoclonal, 10C6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00725-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
10C6
Isotype
Mouse IgG1
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human AKT2, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00725-1 is reactive to AKT2 in Human
Observed Molecular Weight
56 kDa
Calculated molecular weight
55.8 kDa
Background of AKT2
AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. This gene is mapped to 19q13.2. AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00725-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human A549 whole cell, human 293T whole cell, human HELA whole cell, human Caco-2 whole cell, human K562 whole cell, human HL-60 whole cell, human PC-3 whole cell
ICC/IF: MCF7 cell
FCM: A549 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of AKT2 using anti-AKT2 antibody (M00725-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysate,
Lane 2: human 293T whole cell lysate,
Lane 3: human HELA whole cell lysate,
Lane 4: human Caco-2 whole cell lysate,
Lane 5: human K562 whole cell lysate,
Lane 6: human HL-60 whole cell lysate,
Lane 7: human PC-3 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AKT2 antigen affinity purified monoclonal antibody (Catalog # M00725-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Click image to see more details
IF analysis of AKT2 using anti-AKT2 antibody (M00725-1).
AKT2 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-AKT2 Antibody (M00725-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Flow Cytometry analysis of A549 cells using anti-AKT2 antibody (M00725-1).
Overlay histogram showing A549 cells stained with M00725-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AKT2 Antibody (M00725-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-AKT2 Antibody Picoband® (monoclonal, 10C6) (M00725-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-AKT2 Antibody Picoband® (monoclonal, 10C6)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-AKT2 Antibody Picoband® (monoclonal, 10C6)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-AKT2 Antibody Picoband® (monoclonal, 10C6)
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-AKT2 antibody (monoclonal, 10C6) M00725-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-08
Question
Is a blocking peptide available for product anti-AKT2 antibody (monoclonal, 10C6) (M00725-1)?
E. Jones
Verified customer
Asked: 2017-03-01
Answer
We do provide the blocking peptide for product anti-AKT2 antibody (monoclonal, 10C6) (M00725-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-03-01
Question
Do you have a BSA free version of anti-AKT2 antibody (monoclonal, 10C6) M00725-1 available?
B. Krishna
Verified customer
Asked: 2017-03-01
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-AKT2 antibody (monoclonal, 10C6) M00725-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-03-01
