Product Info Summary
SKU: | EK2096 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human Cadherin-6/CDH6 ELISA Kit PicoKine®
View all Cadherin-6/KCAD ELISA kits
SKU/Catalog Number
EK2096
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Cadherin-6/CDH6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CDH6 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Cadherin-6/CDH6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2096)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S54-A615
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2096 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2096 is reactive to CDH6 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2096 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cadherin-6/KCAD
Cadherin-6 is a protein that in humans is encoded by the CDH6 gene. It is mapped to 5p13.3. This gene encodes a member of the cadherin superfamily. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation. Decreased expression of this gene may be associated with tumor growth and metastasis.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK2096-CAP | Anti-Human CDH6 Pre-coated 96-well strip microplate | 1 |
EK2096-ST | Human CDH6 Standard | 2 vials, 10 ng/tube |
EK2096-DA | Human CDH6 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.007 | 0.045 | 0.082 | 0.137 | 0.244 | 0.521 | 1.084 | 1.892 |
Data Example Images
Click image to see more details
Human Cadherin-6 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cadherin-6/KCAD, Dilution ratio of 1:1, concentration in serum and plasma is around 500 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Cadherin-6/CDH6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 188 | 813 | 3740 | 214 | 954 | 4861 |
Standard deviation | 7.71 | 34.96 | 172.04 | 10.91 | 56.29 | 325.69 |
CV (%) | 4.1% | 4.3% | 4.6% | 5.1% | 5.9% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cadherin-6/KCAD in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 188 | 180 | 203 | 210 | 195 | 11.86 | 6.0% |
Sample 2 | 813 | 747 | 779 | 790 | 782 | 23.76 | 3.0% |
Sample 3 | 3740 | 3185 | 3099 | 3077 | 3275 | 271.34 | 8.2% |
Protein Target Info & Infographic
Gene/Protein Information For CDH6 (Source: Uniprot.Org, NCBI)
Gene Name
CDH6
Full Name
Cadherin-6
Weight
88.309kDa
Alternative Names
cadherin 6, type 2, K-cadherin (fetal kidney); cadherin, fetal kidney; Cadherin6; Cadherin-6; CDH6; KCAD; K-cadherin (fetal kidney); K-Cadherin CDH6 CAD6, KCAD cadherin 6 cadherin-6|cadherin 6, type 2, K-cadherin (fetal kidney)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CDH6, check out the CDH6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CDH6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Cadherin-6/CDH6 ELISA Kit PicoKine® (EK2096)
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Customer Q&As
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9 Customer Q&As for Human Cadherin-6/CDH6 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-12-20
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-12-20
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
P. Bhasin
Verified customer
Asked: 2020-11-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-11-22
Question
Q: which procedure should I follow in order to thaw whole blood sample for Cadherin-6 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-07-29
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Cadherin-6 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-07-29
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
P. Kulkarni
Verified customer
Asked: 2019-11-22
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-11-22
Question
Q: if the enzyme conjugated Cadherin-6 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cadherin-6 antigen?
Verified Customer
Verified customer
Asked: 2018-10-20
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-10-20
Question
Q: What is the optimal O.D. value for Cadherin-6 ELISA kit? I performed your Cadherin-6 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Cadherin-6 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Cadherin-6 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Cadherin-6 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2018-05-30
Question
Q: how to analyze ELISA data? I have performed Cadherin-6 level in serum.
Verified Customer
Verified customer
Asked: 2018-05-19
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-05-19
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Cadherin-6 using the EK2096 Human Cadherin-6 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-05-12
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-05-12
Question
Q: can I use citrate plasma as samples in Human Cadherin-6 Picokine® ELISA Kit (Catalog # EK2096)?
G. Roberts
Verified customer
Asked: 2017-02-25
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Cadherin-6 causing disruption of its protein structure. Cadherin-6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Cadherin-6 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-02-25