Anti-ALDH3A1 Antibody

Aldehyde Dehydrogenase 3-A1/ALDH3A1 antibody

Boster Bio Anti-ALDH3A1 Antibody catalog # PA2074. Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA2074
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-ALDH3A1 Antibody

View all Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibodies

SKU/Catalog Number

PA2074

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ALDH3A1 Antibody catalog # PA2074. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ALDH3A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2074)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ALDH3A1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2074 is reactive to ALDH3A1 in Human

Applications

PA2074 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

50.395kDa

Background of Aldehyde Dehydrogenase 3-A1/ALDH3A1

ALDH3A1 (Aldehyde Dehydrogenase, Family 3, Subfamily A, Member 1), also known as ALDH3, is an enzyme that in humans is encoded by the ALDH3A1 gene. By in situ hybridization, Hiraoka et al. (1995) mapped the ALDH3 gene to 17p11.2. ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ALDH3A1 (Source: Uniprot.org, NCBI)

Gene Name

ALDH3A1

Full Name

Aldehyde dehydrogenase, dimeric NADP-preferring

Weight

50.395kDa

Superfamily

aldehyde dehydrogenase family

Alternative Names

aldehyde dehydrogenase 3 family, member A1; Aldehyde dehydrogenase 3; Aldehyde Dehydrogenase 3A1; Aldehyde Dehydrogenase 3-A1; Aldehyde dehydrogenase family 3 member A1; aldehyde dehydrogenase isozyme 3; aldehyde dehydrogenase type III; ALDH3A1; ALDH3aldehyde dehydrogenase, dimeric NADP-preferring; ALDHIII; EC 1.2.1; EC 1.2.1.5; MGC10406; stomach aldehyde dehydrogenase ALDH3A1 ALDH3, ALDHIII aldehyde dehydrogenase 3 family member A1 aldehyde dehydrogenase, dimeric NADP-preferring|aldehyde dehydrogenase isozyme 3|aldehyde dehydrogenase type III|stomach aldehyde dehydrogenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALDH3A1, check out the ALDH3A1 Infographic

ALDH3A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALDH3A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-ALDH3A1 Antibody

Question

Does PA2074 anti-ALDH3A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

As indicated on the product datasheet, PA2074 anti-ALDH3A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-07

Question

We are currently using anti-ALDH3A1 antibody PA2074 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

The anti-ALDH3A1 antibody (PA2074) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-23

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix tongue using anti-ALDH3A1 antibody PA2074. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-11

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-11

Question

Is this PA2074 anti-ALDH3A1 antibody reactive to the isotypes of ALDH3A1?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

The immunogen of PA2074 anti-ALDH3A1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human ALDH3A1(440-453aa KVRYPPSPAKMTQH). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-04

Question

I was wanting to use your anti-ALDH3A1 antibody for WB for human cervix tongue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix tongue identification?

Verified Customer

Verified customer

Asked: 2019-01-31

Answer

You can see on the product datasheet, PA2074 anti-ALDH3A1 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix tongue in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-01-31

Question

Will anti-ALDH3A1 antibody PA2074 work on dog IHC with nasal cavity epithelium?

Verified Customer

Verified customer

Asked: 2018-09-27

Answer

Our lab technicians have not validated anti-ALDH3A1 antibody PA2074 on dog. You can run a BLAST between dog and the immunogen sequence of anti-ALDH3A1 antibody PA2074 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog nasal cavity epithelium in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-27

Size

Conjugation

Total: $370

SKU:PA2074

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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