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Product Info Summary
| SKU: | PA1028 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-C Reactive Protein/CRP Antibody Picoband®
SKU/Catalog Number
PA1028
BA4086-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-C Reactive Protein/CRP Antibody catalog # PA1028. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C Reactive Protein/CRP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1028)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human C Reactive Protein.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1028 is reactive to CRP in Human
Observed Molecular Weight
43 kDa
Calculated molecular weight
25.0 kDa
Background of CRP
C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1028 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC Cell, HT1080 Cell
Validation Images & Assay Conditions
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Anti-C Reactive Protein antibody, PA1028, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: HT1080 Cell Lysate
Specific Publications For Anti-C Reactive Protein/CRP Antibody Picoband® (PA1028)
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Customer Q&As
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4 Customer Q&As for Anti-C Reactive Protein/CRP Antibody Picoband®
Question
My question regards using your anti-C Reactive Protein/CRP antibody for innate immune response studies. Has this antibody been tested with western blotting on smmc cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
I appreciate your inquiry. This PA1028 anti-C Reactive Protein/CRP antibody is tested on smmc cell lysate, ht1080 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-23
Question
My team were content with the WB result of your anti-C Reactive Protein/CRP antibody. However we have seen positive staining in right lobe of liver secreted. using this antibody. Is that expected? Could you tell me where is CRP supposed to be expressed?
J. Krishna
Verified customer
Asked: 2020-01-06
Answer
According to literature, right lobe of liver does express CRP. Generally CRP expresses in secreted. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-06
Question
We have observed staining in human urinary bladder. Any tips? Is anti-C Reactive Protein/CRP antibody supposed to stain urinary bladder positively?
P. Banerjee
Verified customer
Asked: 2019-11-12
Answer
Based on literature urinary bladder does express CRP. Based on Uniprot.org, CRP is expressed in right lobe of liver, liver, urinary bladder, among other tissues. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-12
Question
We are currently using anti-C Reactive Protein/CRP antibody PA1028 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
The anti-C Reactive Protein/CRP antibody (PA1028) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-21
