|Product Name||Anti-C Reactive Protein/CRP Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for C-reactive protein(CRP) detection. Tested with WB in Human.|
|Cite This Product||Anti-C Reactive Protein/CRP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1028)|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human C Reactive Protein(209-224aa KYEVQGEVFTKPQLWP).|
Assay Dilutions Overview
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Images And Assay Conditions
Anti-C Reactive Protein antibody, PA1028, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: HT1080 Cell Lysate
Protein Target Info (Source: Uniprot.org)
|Protein Name||C-reactive protein|
|Tissue Specificity||Found in plasma.|
|Alternative Names||C-reactive protein;C-reactive protein(1-205);CRP;PTX1;|
|Molecular Weight||25039 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.|
|Background||C Reactive Protein(CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.|
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,