Anti-ALK Antibody

ALK/CD246 antibody

Boster Bio Anti-ALK Antibody catalog # PA1741. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1741
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-ALK Antibody

View all ALK/CD246 Antibodies

SKU/Catalog Number

PA1741

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ALK Antibody catalog # PA1741. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ALK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1741)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ALK, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1741 is reactive to ALK in Human, Mouse, Rat

Applications

PA1741 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

176.442kDa

Background of ALK/CD246

ALK (Anaplastic lymphoma kinase) also known as ALK tyrosine kinase receptor or CD246 (cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. The ALK gene is mapped on 2p23.2-p23.1. Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. The deduced amino acid sequences reveal that ALK is a novel receptor tyrosine kinase having a putative transmembrane domain and an extracellular domain. In Drosophila, localized Jeb activates Alk and the downstream Ras/mitogen-activated protein kinase cascade to specify a select group of visceral muscle precursors as muscle-patterning pioneers. Functional RNA interference screening on a set of these transcriptional targets revealed that CEBPB and BCL2A1 were absolutely necessary to induce cell transformation and/or to sustain growth and survival of ALK-positive ALCL cells. One particularly informative case presented a high-level gene amplification that was strictly limited to ALK, indicating that this gene may contribute on its own to neuroblastoma development. Mutated ALK proteins were overexpressed, hyperphosphorylated, and showed constitutive kinase activity. The knockdown of ALK expression in ALK-mutated cells, but also in cell lines overexpressing a wildtype ALK, led to a marked decrease of cell proliferation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For ALK (Source: Uniprot.org, NCBI)

Gene Name

ALK

Full Name

ALK tyrosine kinase receptor

Weight

176.442kDa

Superfamily

protein kinase superfamily

Alternative Names

ALK tyrosine kinase receptor; ALK; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; anaplastic lymphoma receptor tyrosine kinase; CD246 antigen; CD246; EC 2.7.10.1; Ki-1; NBLST3; Tcrz ALK CD246, NBLST3 ALK receptor tyrosine kinase ALK tyrosine kinase receptor|CD246 antigen|anaplastic lymphoma receptor tyrosine kinase|mutant anaplastic lymphoma kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALK, check out the ALK Infographic

ALK infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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17 Customer Q&As for Anti-ALK Antibody

Question

Would anti-ALK antibody PA1741 work on bovine WB with brain?

R. Patel

Verified customer

Asked: 2020-02-12

Answer

Our lab technicians have not tested anti-ALK antibody PA1741 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-ALK antibody PA1741 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-12

Question

Is a blocking peptide available for product anti-ALK antibody (PA1741)?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

We do provide the blocking peptide for product anti-ALK antibody (PA1741). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-03

Question

Does anti-ALK antibody PA1741 work for WB with brain?

A. Johnson

Verified customer

Asked: 2020-01-13

Answer

According to the expression profile of brain, ALK is highly expressed in brain. So, it is likely that anti-ALK antibody PA1741 will work for WB with brain.

Boster Scientific Support

Answered: 2020-01-13

Question

We bought anti-ALK antibody for IHC on brain a few years ago. I am using mouse, and We intend to use the antibody for WB next. you antibody examining brain as well as sperm in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

I looked at the website and datasheets of our anti-ALK antibody and it appears that PA1741 has been validated on mouse in both IHC and WB. Thus PA1741 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-01

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-ALK antibody PA1741. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-04-04

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-04

Question

We have observed staining in human brain. Any tips? Is anti-ALK antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2018-03-19

Answer

Based on literature brain does express ALK. Based on Uniprot.org, ALK is expressed in sperm, brain, among other tissues. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621

Boster Scientific Support

Answered: 2018-03-19

Question

We are interested in to test anti-ALK antibody PA1741 on rat brain for research purposes, then I may be interested in using anti-ALK antibody PA1741 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-02-20

Answer

The products we sell, including anti-ALK antibody PA1741, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-02-20

Question

I was wanting to use your anti-ALK antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?

Verified Customer

Verified customer

Asked: 2017-12-29

Answer

As indicated on the product datasheet, PA1741 anti-ALK antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-12-29

Question

We are currently using anti-ALK antibody PA1741 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

M. Roberts

Verified customer

Asked: 2017-07-26

Answer

The anti-ALK antibody (PA1741) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-26

Question

Is there a BSA free version of anti-ALK antibody PA1741 available?

Verified Customer

Verified customer

Asked: 2017-06-01

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ALK antibody PA1741 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-06-01

Question

My team were content with the WB result of your anti-ALK antibody. However we have observed positive staining in sperm cell membrane using this antibody. Is that expected? Could you tell me where is ALK supposed to be expressed?

J. Carter

Verified customer

Asked: 2016-09-26

Answer

From literature, sperm does express ALK. Generally ALK expresses in cell membrane. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621

Boster Scientific Support

Answered: 2016-09-26

Question

Will PA1741 anti-ALK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

E. Jones

Verified customer

Asked: 2015-08-04

Answer

As indicated on the product datasheet, PA1741 anti-ALK antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-08-04

Question

I see that the anti-ALK antibody PA1741 works with WB, what is the protocol used to produce the result images on the product page?

K. Rodriguez

Verified customer

Asked: 2015-04-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-04-23

Question

Is this PA1741 anti-ALK antibody reactive to the isotypes of ALK?

L. Miller

Verified customer

Asked: 2015-01-29

Answer

The immunogen of PA1741 anti-ALK antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human ALK(1571-1589aa LFRLRHFPCGNVNYGYQQQ), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-01-29

Question

Here is the WB image, lot number and protocol we used for brain using anti-ALK antibody PA1741. Please let me know if you require anything else.

O. Anderson

Verified customer

Asked: 2014-04-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-04-11

Question

I would like using your anti-ALK antibody for protein autophosphorylation studies. Has this antibody been tested with western blotting on rat intestine tissue? We would like to see some validation images before ordering.

M. Li

Verified customer

Asked: 2013-11-05

Answer

We appreciate your inquiry. This PA1741 anti-ALK antibody is tested on rat intestine tissue, brain tissue, tissue lysate, testis tissue, u87 cell lysate, hela cell lysate, colo320 cell lysate, jurkat cell lysate, rectal cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-11-05

Question

I have a question about product PA1741, anti-ALK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Huang

Verified customer

Asked: 2013-07-12

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1741 anti-ALK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-07-12

Size

Conjugation

Total: $370

SKU:PA1741

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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