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Product Info Summary
| SKU: | PA1741 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ALK Antibody Picoband®
SKU/Catalog Number
PA1741
BA3729 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ALK Antibody catalog # PA1741. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1741)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ALK, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1741 is reactive to ALK in Human, Mouse, Rat
Observed Molecular Weight
250 kDa
Calculated molecular weight
176.4 kDa
Background of ALK
ALK (Anaplastic lymphoma kinase) also known as ALK tyrosine kinase receptor or CD246 (cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. The ALK gene is mapped on 2p23.2-p23.1. Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. The deduced amino acid sequences reveal that ALK is a novel receptor tyrosine kinase having a putative transmembrane domain and an extracellular domain. In Drosophila, localized Jeb activates Alk and the downstream Ras/mitogen-activated protein kinase cascade to specify a select group of visceral muscle precursors as muscle-patterning pioneers. Functional RNA interference screening on a set of these transcriptional targets revealed that CEBPB and BCL2A1 were absolutely necessary to induce cell transformation and/or to sustain growth and survival of ALK-positive ALCL cells. One particularly informative case presented a high-level gene amplification that was strictly limited to ALK, indicating that this gene may contribute on its own to neuroblastoma development. Mutated ALK proteins were overexpressed, hyperphosphorylated, and showed constitutive kinase activity. The knockdown of ALK expression in ALK-mutated cells, but also in cell lines overexpressing a wildtype ALK, led to a marked decrease of cell proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1741 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Positive Control
WB: human SH-SY5Y whole cell
IHC: mouse brain tissue, rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of ALK using anti-ALK antibody (PA1741).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALK antigen affinity purified polyclonal antibody (PA1741) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALK at approximately 250 kDa. The expected band size for ALK is at 176 kDa.
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IHC analysis of ALK using anti-ALK antibody (PA1741).
ALK was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALK Antibody (PA1741) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of ALK using anti-ALK antibody (PA1741).
ALK was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALK Antibody (PA1741) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-ALK Antibody Picoband® (PA1741)
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Customer Q&As
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17 Customer Q&As for Anti-ALK Antibody Picoband®
Question
Would anti-ALK antibody PA1741 work on bovine WB with brain?
R. Patel
Verified customer
Asked: 2020-02-12
Answer
Our lab technicians have not tested anti-ALK antibody PA1741 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-ALK antibody PA1741 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-12
Question
Is a blocking peptide available for product anti-ALK antibody (PA1741)?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
We do provide the blocking peptide for product anti-ALK antibody (PA1741). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-03
Question
Does anti-ALK antibody PA1741 work for WB with brain?
A. Johnson
Verified customer
Asked: 2020-01-13
Answer
According to the expression profile of brain, ALK is highly expressed in brain. So, it is likely that anti-ALK antibody PA1741 will work for WB with brain.
Boster Scientific Support
Answered: 2020-01-13
Question
We bought anti-ALK antibody for IHC on brain a few years ago. I am using mouse, and We intend to use the antibody for WB next. you antibody examining brain as well as sperm in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
I looked at the website and datasheets of our anti-ALK antibody and it appears that PA1741 has been validated on mouse in both IHC and WB. Thus PA1741 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-01
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-ALK antibody PA1741. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-04-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-04
Question
We have observed staining in human brain. Any tips? Is anti-ALK antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2018-03-19
Answer
Based on literature brain does express ALK. Based on Uniprot.org, ALK is expressed in sperm, brain, among other tissues. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621
Boster Scientific Support
Answered: 2018-03-19
Question
We are interested in to test anti-ALK antibody PA1741 on rat brain for research purposes, then I may be interested in using anti-ALK antibody PA1741 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-20
Answer
The products we sell, including anti-ALK antibody PA1741, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-20
Question
I was wanting to use your anti-ALK antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
As indicated on the product datasheet, PA1741 anti-ALK antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-29
Question
We are currently using anti-ALK antibody PA1741 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
M. Roberts
Verified customer
Asked: 2017-07-26
Answer
The anti-ALK antibody (PA1741) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-26
Question
Is there a BSA free version of anti-ALK antibody PA1741 available?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ALK antibody PA1741 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-01
Question
My team were content with the WB result of your anti-ALK antibody. However we have observed positive staining in sperm cell membrane using this antibody. Is that expected? Could you tell me where is ALK supposed to be expressed?
J. Carter
Verified customer
Asked: 2016-09-26
Answer
From literature, sperm does express ALK. Generally ALK expresses in cell membrane. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621
Boster Scientific Support
Answered: 2016-09-26
Question
Will PA1741 anti-ALK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Jones
Verified customer
Asked: 2015-08-04
Answer
As indicated on the product datasheet, PA1741 anti-ALK antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-08-04
Question
I see that the anti-ALK antibody PA1741 works with WB, what is the protocol used to produce the result images on the product page?
K. Rodriguez
Verified customer
Asked: 2015-04-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-04-23
Question
Is this PA1741 anti-ALK antibody reactive to the isotypes of ALK?
L. Miller
Verified customer
Asked: 2015-01-29
Answer
The immunogen of PA1741 anti-ALK antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human ALK(1571-1589aa LFRLRHFPCGNVNYGYQQQ), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-01-29
Question
Here is the WB image, lot number and protocol we used for brain using anti-ALK antibody PA1741. Please let me know if you require anything else.
O. Anderson
Verified customer
Asked: 2014-04-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-11
Question
I would like using your anti-ALK antibody for protein autophosphorylation studies. Has this antibody been tested with western blotting on rat intestine tissue? We would like to see some validation images before ordering.
M. Li
Verified customer
Asked: 2013-11-05
Answer
We appreciate your inquiry. This PA1741 anti-ALK antibody is tested on rat intestine tissue, brain tissue, tissue lysate, testis tissue, u87 cell lysate, hela cell lysate, colo320 cell lysate, jurkat cell lysate, rectal cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-11-05
Question
I have a question about product PA1741, anti-ALK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Huang
Verified customer
Asked: 2013-07-12
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1741 anti-ALK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-07-12
