Anti-ALK Rabbit Monoclonal Antibody, Clone#RM361

ALK/CD246 antibody

Boster Bio Anti-ALK Rabbit Monoclonal Antibody, Clone#RM361 (Catalog # M00301-1). Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M00301-1
Size: 100uL
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-ALK Rabbit Monoclonal Antibody, Clone#RM361

View all ALK/CD246 Antibodies

SKU/Catalog Number

M00301-1

Size

100uL

Form

Liquid

Description

Boster Bio Anti-ALK Rabbit Monoclonal Antibody, Clone#RM361 (Catalog # M00301-1). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ALK Rabbit Monoclonal Antibody, Clone#RM361 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00301-1)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM361

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to the C-terminus of human ALK (Anaplastic lymphoma kinase)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts human total ALK protein including ALK fusion proteins

Reactive Species

M00301-1 is reactive to ALK in Human

Applications

M00301-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

176.442kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For ALK (Source: Uniprot.org, NCBI)

Gene Name

ALK

Full Name

ALK tyrosine kinase receptor

Weight

176.442kDa

Superfamily

protein kinase superfamily

Alternative Names

ALK tyrosine kinase receptor; ALK; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; anaplastic lymphoma receptor tyrosine kinase; CD246 antigen; CD246; EC 2.7.10.1; Ki-1; NBLST3; Tcrz ALK CD246, NBLST3 ALK receptor tyrosine kinase ALK tyrosine kinase receptor|CD246 antigen|anaplastic lymphoma receptor tyrosine kinase|mutant anaplastic lymphoma kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALK, check out the ALK Infographic

ALK infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-ALK Rabbit Monoclonal Antibody, Clone#RM361

Question

Can you help my question with product M00301-1, anti-ALK Rabbit Monoclonal antibody, Clone#RM361. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-14

Question

My boss were happy with the WB result of your anti-ALK Rabbit Monoclonal antibody, Clone

R. Li

Verified customer

Asked: 2020-04-02

Answer

According to literature, brain does express ALK. Generally ALK expresses in cell membrane. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621

Boster Scientific Support

Answered: 2020-04-02

Question

I see that the anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-23

Question

We are currently using anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

The anti-ALK Rabbit Monoclonal antibody, Clone#RM361 (M00301-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-20

Question

I was wanting to use your anti-ALK Rabbit Monoclonal antibody, Clone#RM361 for IHC for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

It shows on the product datasheet, M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-31

Question

We bought anti-ALK Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

I have checked the website and datasheets of our anti-ALK Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2019-07-26

Question

Will anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 work for IHC with brain?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

According to the expression profile of brain, ALK is highly expressed in brain. So, it is likely that anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 will work for IHC with brain.

Boster Scientific Support

Answered: 2019-06-27

Question

Is a blocking peptide available for product anti-ALK Rabbit Monoclonal antibody, Clone#RM361 (M00301-1)?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

We do provide the blocking peptide for product anti-ALK Rabbit Monoclonal antibody, Clone#RM361 (M00301-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-21

Question

Is this M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 reactive to the isotypes of ALK?

N. Anderson

Verified customer

Asked: 2019-04-12

Answer

The immunogen of M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 is A peptide corresponding to the C-terminus of human ALK (Anaplastic lymphoma kinase). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-04-12

Question

Do you have a BSA free version of anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 available?

Verified Customer

Verified customer

Asked: 2019-03-21

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-03-21

Question

My lab would like to test anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 on human brain for research purposes, then I may be interested in using anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-12-21

Answer

The products we sell, including anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-12-21

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-06-20

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-20

Question

See attached the WB image, lot number and protocol we used for brain using anti-ALK Rabbit Monoclonal antibody, Clone#RM361 M00301-1. Please let me know if you require anything else.

K. Rodriguez

Verified customer

Asked: 2017-03-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-03-07

Question

Would M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

H. Baker

Verified customer

Asked: 2014-11-10

Answer

It shows on the product datasheet, M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-11-10

Question

We have observed staining in human sperm. Are there any suggestions? Is anti-ALK Rabbit Monoclonal antibody, Clone#RM361 supposed to stain sperm positively?

J. Jackson

Verified customer

Asked: 2013-12-26

Answer

From what I have seen in literature sperm does express ALK. From what I have seen in Uniprot.org, ALK is expressed in sperm, brain, among other tissues. Regarding which tissues have ALK expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15815621

Boster Scientific Support

Answered: 2013-12-26

Question

My question regards using your anti-ALK Rabbit Monoclonal antibody, Clone#RM361 for phosphorylation studies. Has this antibody been tested with western blotting on daudi cells? We would like to see some validation images before ordering.

T. Parker

Verified customer

Asked: 2013-01-14

Answer

We appreciate your inquiry. This M00301-1 anti-ALK Rabbit Monoclonal antibody, Clone#RM361 is validated on daudi cells, human brain tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-01-14

$431
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Lead time for this item is typically 1-2 weeks

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M00301-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$431.00

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