Anti-Alkaline Phosphatase ALPI Antibody

Alkaline Phosphatase, Intestinal antibody

Boster Bio Anti-Alkaline Phosphatase ALPI Antibody (Catalog # P06115). Tested in WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: P06115
Size: 100ug
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Alkaline Phosphatase ALPI Antibody

View all Alkaline Phosphatase, Intestinal Antibodies

SKU/Catalog Number

P06115

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-Alkaline Phosphatase ALPI Antibody (Catalog # P06115). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Alkaline Phosphatase ALPI Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P06115)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Alkaline Phosphatase [Human Intestine]

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P06115 is reactive to ALPI in Human

Applications

P06115 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

56.812kDa

Background of Alkaline Phosphatase, Intestinal

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:4,000 - 1:20,000
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For ALPI (Source: Uniprot.org, NCBI)

Gene Name

ALPI

Full Name

Intestinal-type alkaline phosphatase

Weight

56.812kDa

Superfamily

alkaline phosphatase family

Alternative Names

alkaline phosphatase, intestinal; Alkaline phosphomonoesterase; ALPI; EC 3.1.3.1; Glycerophosphatase; IAP; Intestinal alkaline phosphatase; intestinal-type alkaline phosphatase; Kasahara isozyme ALPI IAP alkaline phosphatase, intestinal intestinal-type alkaline phosphatase|Kasahara isozyme|alkaline phosphomonoesterase|glycerophosphatase|intestinal alkaline phosphatase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALPI, check out the ALPI Infographic

ALPI infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALPI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

P06115 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Synergistic effects of fibroblast growth factor-2 and bone morphogenetic protein-2 on bone induction

Synergistic effects of fibroblast growth factor-2 and bone morphogenetic protein-2 on bone induction

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3 Customer Q&As for Anti-Alkaline Phosphatase ALPI Antibody

Question

We are currently using anti-Alkaline Phosphatase antibody P06115 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2017-11-27

Answer

The anti-Alkaline Phosphatase antibody (P06115) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-27

Question

Will anti-Alkaline Phosphatase antibody P06115 work on canine WB with cervix carcinoma?

S. Wu

Verified customer

Asked: 2016-10-05

Answer

Our lab technicians have not validated anti-Alkaline Phosphatase antibody P06115 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Alkaline Phosphatase antibody P06115 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-10-05

Question

Will P06115 anti-Alkaline Phosphatase antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

P. Wu

Verified customer

Asked: 2015-03-16

Answer

You can see on the product datasheet, P06115 anti-Alkaline Phosphatase antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-03-16

Size

Total: $599

SKU:P06115

Backordered.

Lead time for this item is typically 4-6 days

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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