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Product Info Summary
| SKU: | P06115 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Alkaline Phosphatase ALPI Antibody
SKU/Catalog Number
P06115
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-Alkaline Phosphatase ALPI Antibody (Catalog # P06115). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)
Cite This Product
Anti-Alkaline Phosphatase ALPI Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P06115)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Alkaline Phosphatase [Human Intestine]
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P06115 is reactive to ALP1 in Human
Observed Molecular Weight
52 kDa
Calculated molecular weight
56.8 kDa
Background of ALP1
In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P06115 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:4,000 - 1:20,000
WB: 1:500 - 1:2,000
ALKALINE PHOSPHATASE Antibody has been assayed against 1.0 µg of Alkaline Phosphatase [Human Intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Validation Images & Assay Conditions
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Western Blot of Rabbit Anti-Alkaline Phosphatase Primary Antibody. Molecular Weight Marker. Lane 1: Alkaline Phosphatase. Load: 50 ng per lane. Primary antibody: Alkaline Phosphatase primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: for 30 min at RT. Predicted/Observed size: 58 kDa for Alkaline Phosphatase.
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Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
Specific Publications For Anti-Alkaline Phosphatase ALPI Antibody (P06115)
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3 Customer Q&As for Anti-Alkaline Phosphatase ALPI Antibody
Question
We are currently using anti-Alkaline Phosphatase antibody P06115 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
The anti-Alkaline Phosphatase antibody (P06115) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-27
Question
Will anti-Alkaline Phosphatase antibody P06115 work on canine WB with cervix carcinoma?
S. Wu
Verified customer
Asked: 2016-10-05
Answer
Our lab technicians have not validated anti-Alkaline Phosphatase antibody P06115 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Alkaline Phosphatase antibody P06115 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-10-05
Question
Will P06115 anti-Alkaline Phosphatase antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Wu
Verified customer
Asked: 2015-03-16
Answer
You can see on the product datasheet, P06115 anti-Alkaline Phosphatase antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-03-16
