Anti-Perilipin 3/PLIN3 Antibody Picoband®

Product Name

Anti-Perilipin 3/PLIN3 Antibody Picoband®

View all PLIN3 Antibodies

SKU/Catalog Number

PB10013

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Perilipin 3/PLIN3 Antibody Picoband® catalog # PB10013. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Perilipin 3/PLIN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10013)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Perilipin 3, different from the related mouse sequence by fourteen amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB10013 is reactive to PLIN3 in Human, Mouse, Rat

Observed Molecular Weight

47 kDa

Calculated molecular weight

47.1 kDa

Background of PLIN3

Mannose-6-phosphate receptor binding protein 1 (M6PRBP1), also known as Perilipin 3 (PLN3) or TIP47, is a protein which in humans is encoded by the M6PRBP1 gene. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB10013 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human

Positive Control

WB: human PC-3 whole cell, human THP-1 whole cell, human HepG2 whole cell, human U2OS whole cell
IHC: human colon cancer tissue

Validation Images & Assay Conditions

See full target information PLIN3

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