Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)

alpha Adaptin antibody

Boster Bio Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) catalog # MA1105. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: MA1105
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: WB

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Product Name

Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)

View all alpha Adaptin Antibodies

SKU/Catalog Number

MA1105

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) catalog # MA1105. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1105)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

100/2

Isotype

Mouse IgG2a

Immunogen

AP-2 adaptor polypeptides from bovine brain.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1105 is reactive to AP2A1 in Human, Mouse, Rat

Applications

MA1105 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

108 kDa

Calculated molecular weight

107.546kDa

Background of alpha Adaptin

Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.5-1μg/ml, Human, mouse, rat

Validation Images & Assay Conditions

Gene/Protein Information For AP2A1 (Source: Uniprot.org, NCBI)

Gene Name

AP2A1

Full Name

AP-2 complex subunit alpha-1

Weight

107.546kDa

Superfamily

adaptor complexes large subunit family

Alternative Names

100 kDa coated vesicle protein A; Adapter-related protein complex 2 alpha-1 subunit; Adaptor protein complex AP-2 subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; ADTAAAP2-ALPHA; alpha1-adaptin; Alpha-adaptin A; AP-2 complex subunit alpha-1; CLAPA1adaptin, alpha A; Clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit AP2A1 ADTAA, AP2-ALPHA, CLAPA1 adaptor related protein complex 2 subunit alpha 1 AP-2 complex subunit alpha-1|100 kDa coated vesicle protein A|adapter-related protein complex 2 alpha-1 subunit|adapter-related protein complex 2 subunit alpha-1|adaptin, alpha A|adaptor protein complex AP-2 subunit alpha-1|adaptor related protein complex 2 alpha 1 subunit|alpha-adaptin A|alpha1-adaptin|clathrin assembly protein complex 2 alpha-A large chain|clathrin-associated/assembly/adaptor protein, large, alpha 1|plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AP2A1, check out the AP2A1 Infographic

AP2A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AP2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)

Question

We are currently using anti-Alpha-Adaptin antibody (Monoclonal, 100/2) MA1105 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-06-01

Answer

The anti-Alpha-Adaptin antibody (Monoclonal, 100/2) (MA1105) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-06-01

Order DetailsPrice
MA1105

100μg

$370
MA1105-10ug

10μg sample (liquid)

$99
MA1105-Biotin

100 μg Biotin conjugated

$570
MA1105-Cy3

100 μg Cy3 conjugated

$570
MA1105-Dylight488

100 μg Dylight488 conjugated

$570
MA1105-Dylight550

100 μg Dylight550 conjugated

$570
MA1105-Dylight594

100 μg Dylight594 conjugated

$570
MA1105-FITC

100 μg FITC conjugated

$570
MA1105-HRP

100 μg HRP conjugated

$570
MA1105-APC

100 μg APC conjugated

$670
MA1105-PE

100 μg PE conjugated

$670
MA1105-carrier-free

Carrier Free

$370

More conjugates/formats

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MA1105
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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