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Product Info Summary
| SKU: | MA1105 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)
SKU/Catalog Number
MA1105
BM1566 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) catalog # MA1105. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1105)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
100/2
Isotype
Mouse IgG2a
Immunogen
AP-2 adaptor polypeptides from bovine brain.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1105 is reactive to AP2A1 in Human, Mouse, Rat
Observed Molecular Weight
108 kDa
Background of AP2A1
Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1105 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.5-1μg/ml, Human, mouse, rat
Positive Control
WB: human SH-SY5Y whole cell, human U-87MG whole cell, human MCF-7 whole cell, human 293T whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of α-Adaptin using anti-α-Adaptin antibody (MA1105).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-α-Adaptin antigen affinity purified monoclonal antibody (Catalog # MA1105) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for α-Adaptin at approximately 108 kDa. The expected band size for α-Adaptin is at 108 kDa.
Specific Publications For Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) (MA1105)
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1 Customer Q&As for Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)
Question
We are currently using anti-Alpha-Adaptin antibody (Monoclonal, 100/2) MA1105 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
The anti-Alpha-Adaptin antibody (Monoclonal, 100/2) (MA1105) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-01
