Anti-Alpha-Adaptin Antibody (Monoclonal, 100/2)

SKU MA1105
Size 100μg/vial
Reactivity Human, Rat
Clonality Monoclonal
Clone Number 100/2
Host Mouse
Ig Isotype Mouse IgG2a
Applications WB

Overview

Product Name Anti-Alpha-Adaptin Antibody (Monoclonal, 100/2)
SKU/Catalog Number MA1105
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for alpha-Adaptin, adaptor-related protein complex 2, alpha 1 subunit (AP2A1 ) detection. Tested with WB in Human;rat. No cross reactivity with other proteins.
Cite This Product Anti-Alpha-Adaptin Antibody (Monoclonal, 100/2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1105)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen AP-2 adaptor polypeptides from bovine brain.
Reactivity Human, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Western blot, 2-4μg/ml, Human, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Images And Assay Conditions

Boster Kit Box

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id D3ZUY8
Gene Name AP2A1
Protein Name adaptor-related protein complex 2, alpha 1 subunit
Tissue Specificity AP2A1: Isoform A expressed in forebrain, skeletalmuscle, spinal cord, cerebellum, salivary gland, heart and colon.Isoform B is widely expressed in tissues and also in breast cancerand in prostate carcinoma cells.
Alternative Names Adaptor protein complex AP-2, alpha 1 subunit (Predicted) ;Protein Ap2a1 ;Ap2a1 ;Ap2a1_predicted ;rCG_54084 ;
Subcellular Localization AP2A1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV.|Ap2a1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV.
Molecular Weight 107675 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function AP2A1: Component of the adaptor protein complex 2 (AP-2).Adaptor protein complexes function in protein transport viatransport vesicles in different membrane traffic pathways. Adaptorprotein complexes are vesicle coat components and appear to beinvolved in cargo selection and vesicle formation. AP-2 isinvolved in clathrin-dependent endocytosis in which cargo proteinsare incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with theearly endosome. The clathrin lattice serves as a mechanicalscaffold but is itself unable to bind directly to membranecomponents. Clathrin-associated adaptor protein (AP) complexeswhich can bind directly to both the clathrin lattice and to thelipid and protein components of membranes are considered to be themajor clathrin adaptors contributing the CCV formation. AP-2 alsoserves as a cargo receptor to selectively sort the membraneproteins involved in receptor-mediated endocytosis. AP-2 seems toplay a role in the recycling of synaptic vesicle membranes fromthe presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi)and [ED]-X-X-X-L-[LI] endocytosis signal motifs within thecytosolic tails of transmembrane cargo molecules. AP-2 may alsoplay a role in maintaining normal post-endocytic traffickingthrough the ARF6-regulated, non-clathrin pathway. The AP-2 alphasubunit binds polyphosphoinositide-containing lipids, positioningAP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocyticaccessory proteins. The AP-2 alpha and AP-2 sigma subunits arethought to contribute to the recognition of the [ED]-X-X-X-L-[LI]motif (By similarity).
Research Areas Human, Rat

*You can search these to find other products in these research areas.
Background Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1(HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al.(2001) identified 3 HIP1-associated proteins, clathrin heavy chain(CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD(adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.

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Monoclonal clone# G2 antibody for ALPHA ADAPTIN/AP2A1 detection. Host: Mouse.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. ALPHA ADAPTIN/AP2A1 information: Molecular Weight: 107675 MW; Subcellular Localization: AP2A1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV.|Ap2a1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV; Tissue Specificity: AP2A1: Isoform A expressed in forebrain, skeletalmuscle, spinal cord, cerebellum, salivary gland, heart and colon.Isoform B is widely expressed in tissues and also in breast cancerand in prostate carcinoma cells.
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MA1105
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
  • Q: What are some alternative names that could be used to describe this product?
    A: One other very common name is ap2 alpha antibody
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