Product Info Summary
SKU: | MA1105 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | WB |
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Product info
Product Name
Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)
View all alpha Adaptin Antibodies
SKU/Catalog Number
MA1105
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) catalog # MA1105. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1105)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
100/2
Isotype
Mouse IgG2a
Immunogen
AP-2 adaptor polypeptides from bovine brain.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1105 is reactive to AP2A1 in Human, Mouse, Rat
Applications
MA1105 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
108 kDa
Calculated molecular weight
107.546kDa
Background of alpha Adaptin
Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.5-1μg/ml, Human, mouse, rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of α-Adaptin using anti-α-Adaptin antibody (MA1105).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-α-Adaptin antigen affinity purified monoclonal antibody (Catalog # MA1105) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for α-Adaptin at approximately 108 kDa. The expected band size for α-Adaptin is at 108 kDa.
Protein Target Info & Infographic
Gene/Protein Information For AP2A1 (Source: Uniprot.org, NCBI)
Gene Name
AP2A1
Full Name
AP-2 complex subunit alpha-1
Weight
107.546kDa
Superfamily
adaptor complexes large subunit family
Alternative Names
100 kDa coated vesicle protein A; Adapter-related protein complex 2 alpha-1 subunit; Adaptor protein complex AP-2 subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; ADTAAAP2-ALPHA; alpha1-adaptin; Alpha-adaptin A; AP-2 complex subunit alpha-1; CLAPA1adaptin, alpha A; Clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit AP2A1 ADTAA, AP2-ALPHA, CLAPA1 adaptor related protein complex 2 subunit alpha 1 AP-2 complex subunit alpha-1|100 kDa coated vesicle protein A|adapter-related protein complex 2 alpha-1 subunit|adapter-related protein complex 2 subunit alpha-1|adaptin, alpha A|adaptor protein complex AP-2 subunit alpha-1|adaptor related protein complex 2 alpha 1 subunit|alpha-adaptin A|alpha1-adaptin|clathrin assembly protein complex 2 alpha-A large chain|clathrin-associated/assembly/adaptor protein, large, alpha 1|plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AP2A1, check out the AP2A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AP2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) (MA1105)
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No publications found for MA1105
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)
Question
We are currently using anti-Alpha-Adaptin antibody (Monoclonal, 100/2) MA1105 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
The anti-Alpha-Adaptin antibody (Monoclonal, 100/2) (MA1105) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-01