AP2A1 Antibodies

AP-2 complex subunit alpha-1 (AP2A1)

Part of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways.

Adaptor protein complexes are vesicle coat components and seem to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis where cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) that are destined for fusion with the early endosome.

Gene Information

Human
Gene Name:	AP2A1
Uniprot:	O95782
Entrez:	160

Family

Belongs to:
adaptor complexes large subunit family

Alternative Names

100 kDa coated vesicle protein A; Adapter-related protein complex 2 alpha-1 subunit; Adaptor protein complex AP-2 subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; ADTAAAP2-ALPHA; alpha1-adaptin; Alpha-adaptin A; AP-2 complex subunit alpha-1; CLAPA1adaptin, alpha A; Clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

Molecular Weight

Mass (kDA):
107.546 kDA

Genomic Context

Human
Location:	19q13.33
Sequence:	19; NC_000019.10 (49767001..49807114)

Tissue Specificity

Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.

Subcellular Localizations

Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.

Diseases

• Immunologic Deficiency Syndromes
• Hiv Infections
• Rhabdomyosarcoma
• Glioma
• Enterovirus Infections

Pathways

• Endocytosis
• Clathrin-mediated Endocytosis
• Transport
• Notch Signaling Pathway
• Cell Proliferation
• Mitosis
• Pathogenesis
• Viral Replication

• Localization
• Nuclear Transport

PTMs

• Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/AP2A1

Boster Bio - Best Uses Of The AP2A1 Marker

In this article, we will discuss AP2A1 marker and its uses. Boster Bio is an anti-microbial manufacturing company. Their signature products are ELISA kit and antibodies. Recently, they started providing PCR-related molecular biology products as well. Boster Bio also offers services to scientists. They offer technical assistance 24 hours a week and provide free resources to customers.

Gene infographics

The human genome hosts the AP2A1 Gene. This gene can be associated with five thousand ninety-seven and thirty seven biological entities. Researchers can find out more about this gene using gene infographics. The gene has five,900, three hundred and thirty-three functional relationships with biological entities. In this infographic, the AP2A1 gene is visualized in a unique way.

Primary antibodies with high-affinity

The AP2A1 gene is a protein that binds to antigens and AP2A1 is a good candidate for such a specificity. It is not always desirable for a protein to have a high affinity towards antigens. This makes the separation of antigen and antibody more difficult. Therefore, high-affinity primary antibodies using the AP2A1 marker are highly useful in immunohistochemistry.

AP2A1, a common protein found on human cells' surfaces, is a common one. Many vesicles contain multiple epitopes which allow high-affinity primaries to recognize them. Table 3 and 4 provide examples of antigens. See Examples for more details. In the examples, several antigens are selected and conjugated to the antibody arrays.

Purification of anti-IFN-g scFv was performed by Ni2+ affinity chromatography. These steps were similar to protein purification. The purified scFv products were tested for activity using ELISA. IFN-g antigen was coated onto 96-well plates overnight at 4degC. Antibodies were then added to the reaction wells, followed by addition of anti-MBP tag antibodies. Finally, HRP-conjugated goat IgG antibody against anti-mouse was used to detect the purified SCFv products.

Immunoassays based on enzyme-linked immunosorbent assays are also available. These methods can be based on monoclonal or polyclonal antibodies of varying specificity and affinity. The AP2A1 recombinant IFN–g marker shows high affinity. These tests are highly accurate and can provide a better understanding of the immune responses of patients with certain types of cancer.

Multiple studies have demonstrated high-affinity nature in these vesicle detectors. In these studies, plasma samples were taken from prostate cancer patients as well as from healthy controls. The resulting plasma-derived complexes were sorted by antibodies against Argonaute 2 or PCSA. These double positive events were further quantified through flow cytometry. Cells displaying double-positive PCSA/Ago2 events were isolated to isolate RNA.

To study the effect of IFNg on the immune system, immunoassays using IFNg detection have been employed. This antibody is able to detect the antibody to IFN-g without the need to stimulate the immune systems. Moreover, the antibody can be used to perform bio-panning to identify the specific scFv antibody against the IFN-g protein.

AP2A1 marker

The AP2A1 gene is responsible for determining the presence of the AP2 protein in the blood. It is also a marker for RNAi and other signaling pathways. This gene is used in a variety applications and research studies by many people. Boster Bio provides high-affinity primary antibodies and reagents to detect AP2A1. These antibodies are well-validated for use in Western blotting, immunohistochemistry, and ELISA.