Product Info Summary
SKU: | PA1486 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Alpha Amylase 1/AMY1A Antibody
View all Salivary Amylase Alpha Antibodies
SKU/Catalog Number
PA1486
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha Amylase 1/AMY1A Antibody catalog # PA1486. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha Amylase 1/AMY1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1486)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha Amylase 1, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1486 is reactive to AMY1A in Human, Mouse, Rat
Applications
PA1486 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
55-58 kDa
Calculated molecular weight
57.768kDa
Background of Salivary Amylase Alpha
Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylase is present in human saliva, where it begins the chemical process of digestion. By in situ hybridization combined with high resolution cytogenetics, the amylase gene is mapped to 1p21.Amylase enzymes find use in bread making and to break down complex sugars such as starch (found in flour) into simple sugars. Yeast then feeds on these simple sugars and converts it into the waste products of alcohol and CO2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Alpha Amylase 1 using anti-Alpha Amylase 1 antibody (PA1486).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PANC-1 whole cell lysates,
Lane 2: rat pancreas tissue lysates,
Lane 3: mouse pancreas tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Amylase 1 antigen affinity purified polyclonal antibody (Catalog # PA1486) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Amylase 1 at approximately 55-58KD. The expected band size for Alpha Amylase 1 is at 58KD.
Click image to see more details
Figure 2. IHC analysis of Alpha Amylase 1 using anti-Alpha Amylase 1 antibody (PA1486).
Alpha Amylase 1 was detected in paraffin-embedded section of Rat Spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Alpha Amylase 1 Antibody (PA1486) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For AMY1A (Source: Uniprot.org, NCBI)
Gene Name
AMY1A
Full Name
Alpha-amylase 1
Weight
57.768kDa
Superfamily
glycosyl hydrolase 13 family
Alternative Names
1,4-alpha-D-glucan glucanohydrolase 1; alpha-amylase 1; AMY1; AMY1B; AMY1C; amylase, alpha 1A (salivary); amylase, alpha 1A; salivary; amylase, salivary, alpha-1A; EC 3.2.1.1; glycogenase; Salivary alpha-amylase; salivary amylase alpha 1A AMY1A AMY1 amylase alpha 1A alpha-amylase 1A|1,4-alpha-D-glucan glucanohydrolase 1|alpha-amylase 1|amylase alpha 1A (salivary)|amylase, salivary, alpha-1A|glycogenase|salivary alpha-amylase|salivary amylase alpha 1A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AMY1A, check out the AMY1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AMY1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha Amylase 1/AMY1A Antibody (PA1486)
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No publications found for PA1486
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Customer Q&As
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1 Customer Q&As for Anti-Alpha Amylase 1/AMY1A Antibody
Question
We are currently using anti-Alpha Amylase 1/AMY1A antibody PA1486 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
The anti-Alpha Amylase 1/AMY1A antibody (PA1486) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-22