Anti-Alpha Amylase 1/AMY1A Antibody

Salivary Amylase Alpha antibody

Boster Bio Anti-Alpha Amylase 1/AMY1A Antibody catalog # PA1486. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1486
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Alpha Amylase 1/AMY1A Antibody

View all Salivary Amylase Alpha Antibodies

SKU/Catalog Number

PA1486

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha Amylase 1/AMY1A Antibody catalog # PA1486. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha Amylase 1/AMY1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1486)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha Amylase 1, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1486 is reactive to AMY1A in Human, Mouse, Rat

Applications

PA1486 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

55-58 kDa

Calculated molecular weight

57.768kDa

Background of Salivary Amylase Alpha

Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylase is present in human saliva, where it begins the chemical process of digestion. By in situ hybridization combined with high resolution cytogenetics, the amylase gene is mapped to 1p21.Amylase enzymes find use in bread making and to break down complex sugars such as starch (found in flour) into simple sugars. Yeast then feeds on these simple sugars and converts it into the waste products of alcohol and CO2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For AMY1A (Source: Uniprot.org, NCBI)

Gene Name

AMY1A

Full Name

Alpha-amylase 1

Weight

57.768kDa

Superfamily

glycosyl hydrolase 13 family

Alternative Names

1,4-alpha-D-glucan glucanohydrolase 1; alpha-amylase 1; AMY1; AMY1B; AMY1C; amylase, alpha 1A (salivary); amylase, alpha 1A; salivary; amylase, salivary, alpha-1A; EC 3.2.1.1; glycogenase; Salivary alpha-amylase; salivary amylase alpha 1A AMY1A AMY1 amylase alpha 1A alpha-amylase 1A|1,4-alpha-D-glucan glucanohydrolase 1|alpha-amylase 1|amylase alpha 1A (salivary)|amylase, salivary, alpha-1A|glycogenase|salivary alpha-amylase|salivary amylase alpha 1A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AMY1A, check out the AMY1A Infographic

AMY1A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AMY1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Alpha Amylase 1/AMY1A Antibody

Question

We are currently using anti-Alpha Amylase 1/AMY1A antibody PA1486 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-01-22

Answer

The anti-Alpha Amylase 1/AMY1A antibody (PA1486) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-01-22

Size

Conjugation

Total: $370

SKU:PA1486

In stock, 3+ left.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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