Anti-Placental alkaline phosphatase (PLAP)/ALPP Antibody Picoband™

Alkaline Phosphatase/ALPP antibody

Boster Bio Anti-Placental alkaline phosphatase (PLAP)/ALPP Antibody Picoband™ catalog # A01718. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: A01718
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Placental alkaline phosphatase (PLAP)/ALPP Antibody Picoband™

View all Alkaline Phosphatase/ALPP Antibodies

SKU/Catalog Number

A01718

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Placental alkaline phosphatase (PLAP)/ALPP Antibody Picoband™ catalog # A01718. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Placental alkaline phosphatase (PLAP)/ALPP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01718)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ALPP.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A01718 is reactive to ALPP in Human

Applications

A01718 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

57.954kDa

Background of Alkaline Phosphatase/ALPP

Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ALPP (Source: Uniprot.org, NCBI)

Gene Name

ALPP

Full Name

Alkaline phosphatase, placental type

Weight

57.954kDa

Superfamily

alkaline phosphatase family

Alternative Names

Alkaline phosphatase Regan isozyme; Alkaline Phosphatase; alkaline phosphatase, placental (Regan isozyme); alkaline phosphatase, placental type; alkaline phosphatase, placental; alkaline phosphomonoesterase; ALP; ALPP; EC 3.1.3.1; FLJ61142; glycerophosphatase; PALP; Placental alkaline phosphatase 1; PLAP; PLAP-1; Regan isozyme; SEAP ALPP ALP, ALPI, IAP, PALP, PLAP, PLAP-1 alkaline phosphatase, placental alkaline phosphatase, placental type|Intestinal alkaline phosphatase|Intestinal-type alkaline phosphatase|alkaline phosphatase Regan isozyme|alkaline phosphomonoesterase|glycerophosphatase|placental alkaline phosphatase 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALPP, check out the ALPP Infographic

ALPP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALPP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01718 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels
Species: Human

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4 Customer Q&As for Anti-Placental alkaline phosphatase (PLAP)/ALPP Antibody Picoband™

Question

My colleagues were happy with the WB result of your anti-Placental alkaline phosphatase (PLAP)/ALPP antibody. However we have been able to see positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is ALPP supposed to be expressed?

N. Jones

Verified customer

Asked: 2020-02-24

Answer

According to literature, placenta does express ALPP. Generally ALPP expresses in cell membrane. Regarding which tissues have ALPP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix, and Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-02-24

Question

We are currently using anti-Placental alkaline phosphatase (PLAP)/ALPP antibody A01718 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

The anti-Placental alkaline phosphatase (PLAP)/ALPP antibody (A01718) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-05

Question

We bought anti-Placental alkaline phosphatase (PLAP)/ALPP antibody for IHC on cervix carcinoma a few months ago. I am using human, and We are going to use the antibody for WB next. We need examining cervix carcinoma as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-10-25

Answer

I have checked the website and datasheets of our anti-Placental alkaline phosphatase (PLAP)/ALPP antibody and it appears that A01718 has been validated on human in both IHC and WB. Thus A01718 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-10-25

Question

We have been able to see staining in human cervix carcinoma. Do you have any suggestions? Is anti-Placental alkaline phosphatase (PLAP)/ALPP antibody supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2017-06-15

Answer

Based on literature cervix carcinoma does express ALPP. Based on Uniprot.org, ALPP is expressed in placenta, cervix placenta, cervix carcinoma, among other tissues. Regarding which tissues have ALPP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix, and Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-06-15

Size

Conjugation

Total: $370

SKU:A01718

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