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Product Info Summary
| SKU: | A00685-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-MCU Antibody Picoband®
SKU/Catalog Number
A00685-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCU Antibody Picoband® catalog # A00685-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCU Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00685-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MCU recombinant protein (Position: V51-D351).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00685-1 is reactive to MCU in Human, Monkey, Mouse, Rat
Observed Molecular Weight
34 kDa
Calculated molecular weight
39.9 kDa
Background of MCU
The mitochondrial calcium uniporter (MCU) is a transmembrane protein that allows the passage of calcium ions from a cell's cytosol into mitochondria. Its activity is regulated by MICU1 and MICU2, which together with the MCU make up the mitochondrial calcium uniporter complex. This gene encodes a calcium transporter that localizes to the mitochondrial inner membrane. The encoded protein interacts with mitochondrial calcium uptake 1. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00685-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HELA whole cell, human A549 whole cell, human PANC-1 whole cell, monkey COS-7 whole cell, human HL-60 whole cell, human HEPG2 whole cell, human Jurkat whole cell, rat brain tissue, rat kidney tissue, rat heart tissue, rat PC-12 whole cell, mouse brain tissue, mouse kidney tissue, mouse heart tissue, mouse NIH/3T3 whole cell
IHC: mouse lung tissue, rat lung tissue, human liver cancer tissue, human bladder cancer tissue, human bladder cancer tissue
FCM: HELA cell
Validation Images & Assay Conditions
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Western blot analysis of MCU using anti-MCU antibody (A00685-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: human HL-60 whole cell lysates,
Lane 6: human HEPG2 whole cell lysates,
Lane 7: human Jurkat whole cell lysates,
Lane 8: rat brain tissue lysates,
Lane 9: rat kidney tissue lysates,
Lane 10: rat heart tissue lysates,
Lane 11: rat PC-12 whole cell lysates,
Lane 12: mouse brain tissue lysates,
Lane 13: mouse kidney tissue lysates,
Lane 14: mouse heart tissue lysates,
Lane 15: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCU antigen affinity purified polyclonal antibody (Catalog # A00685-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCU at approximately 34KD. The expected band size for MCU is at 34KD.
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IHC analysis of MCU using anti-MCU antibody (A00685-1).
MCU was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MCU Antibody (A00685-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of MCU using anti-MCU antibody (A00685-1).
MCU was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MCU Antibody (A00685-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of MCU using anti-MCU antibody (A00685-1).
MCU was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MCU Antibody (A00685-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of MCU using anti-MCU antibody (A00685-1).
MCU was detected in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MCU Antibody (A00685-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of MCU using anti-MCU antibody (A00685-1).
MCU was detected in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MCU Antibody (A00685-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Flow Cytometry analysis of HELA cells using anti-MCU antibody (A00685-1).
Overlay histogram showing HELA cells stained with A00685-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCU Antibody (A00685-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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CRSJG protects the structure and function of MAMs in MPP + induced nerve cells. After treating with 1,000 μmol/L MPP + -induced SH-SY5Y cells with 2.5% CRSJG-medicated serum or 100 μmol/L 2-APB for 24 h, (A) The microscopic structure of MAMs was observed by transmission electron microscope, magnification (×20,000), magnification of red rectangle box (×,100,000); (B) Western blot was used to detect the expression of major proteins in the Ca 2+ transport complex; (C) IP3R; (D) VDAC; (E) MCU; (F) Grp75. Results are from three independent experiments. M: # P < 0.05, ## P < 0.01 vs. control group; * P < 0.05, ** P < 0.01 vs. MPP + group.
Index in Frontiersin under a CC BY license. DOI: 10.3389/fphar.2025.1509317
Specific Publications For Anti-MCU Antibody Picoband® (A00685-1)
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