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ADAMTS5 antibody (A02802-1) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 23 Publications

a02802 1 adamts5 primary antibodies ihc testing 2

IHC analysis of ADAMTS5 using anti-ADAMTS5 antibody (A02802-1).
ADAMTS5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADAMTS5 Antibody (A02802-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Anti-ADAMTS5 Antibody Picoband®

ADAMTS5 antibody

23 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, IHC, WB

Tested Applications

ADAMTS5 antibody was positively detected by WB in:

human Hela whole cell, human HepG2 whole cell, human U20S whole cell, human SW620 whole cell, human THP-1 whole cell, human Raji whole cell, human SGC-7901 whole cell, rat liver tissue, rat testis tissue, mouse liver tissue, mouse testis tissue,

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ADAMTS5 antibody was positively detected by IHC in:

human placenta tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-ADAMTS5 Antibody Picoband®
View all ADAMTS5 Antibodies

Form

Lyophilized

Uniprot

Q9UNA0

Gene ID

11096

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ADAMTS5 recombinant protein (Position: D747-K780).

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

75 kDa

Calculated Molecular Weight

101.7 kDa

Background of ADAMTS5

ADAMTS5 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 5), is an enzyme that in humans is encoded by the ADAMTS5 gene. ADAMTS5 is a member of the large ADAMTS family of zinc-dependent proteases. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. By somatic cell hybrid analysis, the human ADAMTS5 gene is mapped to chromosome 21. Used mouse models, it is showed that Sdc4 controls a pathway that activates Adamts5 at the chondrocyte cell surface through Erk1/Erk2 activation of Mmp3.

Cite This Product

Anti-ADAMTS5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02802-1)

Protocols

Western Blot protocol for ADAMTS5 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for ADAMTS5 antibody

Review Protocol

Publications

Inhibition of PARP-1 attenuates osteoarthritis progression by suppressing NF-κB signaling and alleviating chondrocyte senescence

Published: 2026-08-22

Journal: CELLULAR SIGNALLING

Application: WB

Impact Factor: 4.70

Species: Rat

PMID: 42632530

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Anti-ADAMTS5 Antibody Picoband®

ADAMTS5 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Inhibition of PARP-1 attenuates osteoarthritis progression by suppressing NF-κB signaling and alleviating chondrocyte senescence

Denosumab attenuates knee osteoarthritis progression by inhibiting synovial inflammation via the RANK/TRAF6/FSTL1 signalling

Loss of ADAMTS5 promotes vascular calcification via versican/integrin β1/FAK signal

Self-healing hydrogels loaded with Spatholobi Caulis alleviate disc degeneration by promoting autophagy in nucelus pulposus

TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway

Lipoxin A4 modulates the PKA/CREB and NF-κB signaling pathway to mitigate chondrocyte catabolism and apoptosis in temporomandibular joint osteoarthritis

TBK1 pharmacological inhibition mitigates osteoarthritis through attenuating inflammation and cellular senescence in chondrocytes

Total glucosides of white paeony capsule alleviate articular cartilage degeneration and aberrant subchondral bone remodeling in knee osteoarthritis

Fisetin suppresses chondrocyte senescence and attenuates osteoarthritis progression by targeting sirtuin 6

Identification of several inflammation-related genes based on bioinformatics and experiments

Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments

Mulberroside A alleviates osteoarthritis via restoring impaired autophagy and suppressing MAPK/NF-κB/PI3K-AKT-mTOR signaling pathways

Physalin A Inhibits MAPK and NF-κB Signal Transduction Through Integrin αVβ3 and Exerts Chondroprotective Effect

MCC950, the NLRP3 Inhibitor, Protects against Cartilage Degradation in a Mouse Model of Osteoarthritis

Selective STAT3 Inhibitor Alantolactone Ameliorates Osteoarthritis via Regulating Chondrocyte Autophagy and Cartilage Homeostasis

Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats

Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis

p-Coumaric Acid Attenuates IL-1β-Induced Inflammatory Responses and Cellular Senescence in Rat Chondrocytes

Vanillic acid attenuates cartilage degeneration by regulating the MAPK and PI3K/AKT/NF-κB pathways

Liquiritigenin inhibits IL-1β-induced inflammation and cartilage matrix degradation in rat chondrocytes

Validation of the Diagnostic and Prognostic Values of ADAMTS5 and FSTL1 in Osteoarthritis Rat Model

Schisandrin A Inhibits the IL-1β-Induced Inflammation and Cartilage Degradation via Suppression of MAPK and NF-κB Signal Pathways in Rat Chondrocytes

Sinapic Acid Inhibits the IL-1β-Induced Inflammation via MAPK Downregulation in Rat Chondrocytes

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