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Product Info Summary
| SKU: | A00420-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MMP13 Antibody Picoband®
SKU/Catalog Number
A00420-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MMP13 Antibody Picoband® catalog # A00420-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00420-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat MMP13 recombinant protein (Position: Y99-H335).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00420-2 is reactive to Mmp13 in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
53.4 kDa
Background of Mmp13
Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00420-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human A549 whole cell, human PC-3 whole cell, human U2OS whole cell, human HEK293 whole cell, rat testicular tissue, mouse testicular tissue,
IHC: mouse lung tissue, mouse small intestine tissue, rat lung tissue, rat small intestine tissue
Validation Images & Assay Conditions
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Western blot analysis of MMP13 using anti-MMP13 antibody (A00420-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates
Lane 2: human PC-3 whole cell lysates
Lane 3: human U2OS whole cell lysates
Lane 4: human HEK293 whole cell lysates
Lane 5: rat testicular tissue lysates
Lane 6: mouse testicular tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP13 antigen affinity purified polyclonal antibody (Catalog # A00420-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP13 at approximately 54KD. The expected band size for MMP13 is at 54KD.
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Therapeutic effectiveness evaluated after administering AE@SiO 2 -MTX. (A) Knee joints were stained with HE and safranin O. Scale bar: 100 μm. (B) TNF-α, IL-6, MMP3 and MMP13 and DAPI staining applied to knee sections. (C) Line profile analysis verified the co-expression and co-localization of TNF-α and IL-6, MMP3 and MMP13. Scale bar: 50 μm.
Index in PubMed under a CC BY license. PMID: 40521182
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(A) Representative immunohistochemistry images of ColII and MMP-13 staining across all groups. (B, C) Quantitative analysis of ColII and MMP-13 protein expression levels in the NP. Immunohistological analysis showing that the protein expression level of ColII in the ILS+STZ+ IP group was higher and this of MMP-13 was lower than those in the ILS+STZ group. Note: *P < 0.05, **P < 0.01; scale bars = 200 μm as indicated.
Index in PubMed under a CC BY license. PMID: 40837020
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IHC analysis of MMP13 using anti-MMP13 antibody (A00420-2).
MMP13 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP13 Antibody (A00420-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of MMP13 using anti-MMP13 antibody (A00420-2).
MMP13 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP13 Antibody (A00420-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of MMP13 using anti-MMP13 antibody (A00420-2).
MMP13 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP13 Antibody (A00420-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of MMP13 using anti-MMP13 antibody (A00420-2).
MMP13 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP13 Antibody (A00420-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-MMP13 Antibody Picoband® (A00420-2)
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Customer Q&As
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16 Customer Q&As for Anti-MMP13 Antibody Picoband®
Question
Here is the WB image, lot number and protocol we used for esophagus synovium using anti-MMP13 antibody A00420-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-06
Question
Will A00420-2 anti-MMP13 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
It shows on the product datasheet, A00420-2 anti-MMP13 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-17
Question
We purchased anti-MMP13 antibody for WB on tibia a few months ago. I am using human, and We are going to use the antibody for IHC next. We want examining tibia as well as mammary carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
I took a look at the website and datasheets of our anti-MMP13 antibody and it seems that A00420-2 has been validated on human in both WB and IHC. Thus A00420-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-11
Question
I was wanting to use using your anti-MMP13 antibody for r-rno-2022090; assembly of collagen fibrils and other multimeric structures studies. Has this antibody been tested with western blotting on human a549? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
We appreciate your inquiry. This A00420-2 anti-MMP13 antibody is validated on human a549, a549 whole cell lysates, u2os whole cell lysates, rat lung tissue, mouse lung tissue, small intestine tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-26
Question
I see that the anti-MMP13 antibody A00420-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-19
Question
Can you help my question with product A00420-2, anti-MMP13 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00420-2 anti-MMP13 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-16
Question
Is this A00420-2 anti-MMP13 antibody reactive to the isotypes of MMP13?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
The immunogen of A00420-2 anti-MMP13 antibody is E. coli-derived rat MMP13 recombinant protein (Position: Y99-H335). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-14
Question
We have observed staining in human lung. Are there any suggestions? Is anti-MMP13 antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
Based on literature lung does express MMP13. Based on Uniprot.org, MMP13 is expressed in tibia, mammary carcinoma, esophagus synovium, lung, among other tissues. Regarding which tissues have MMP13 expression, here are a few articles citing expression in various tissues:
Esophagus, and Synovium, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Mammary carcinoma, Pubmed ID: 8207000, 9562863
Boster Scientific Support
Answered: 2019-06-25
Question
We are currently using anti-MMP13 antibody A00420-2 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The anti-MMP13 antibody (A00420-2) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-11
Question
Is a blocking peptide available for product anti-MMP13 antibody (A00420-2)?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
We do provide the blocking peptide for product anti-MMP13 antibody (A00420-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-01-31
Question
We need to test anti-MMP13 antibody A00420-2 on mouse esophagus synovium for research purposes, then I may be interested in using anti-MMP13 antibody A00420-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
The products we sell, including anti-MMP13 antibody A00420-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus synovium using anti-MMP13 antibody A00420-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-14
Question
Would anti-MMP13 antibody A00420-2 work for IHC with esophagus synovium?
C. Miller
Verified customer
Asked: 2016-08-17
Answer
According to the expression profile of esophagus synovium, MMP13 is highly expressed in esophagus synovium. So, it is likely that anti-MMP13 antibody A00420-2 will work for IHC with esophagus synovium.
Boster Scientific Support
Answered: 2016-08-17
Question
I was wanting to use your anti-MMP13 antibody for IHC for mouse esophagus synovium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse esophagus synovium identification?
J. Singh
Verified customer
Asked: 2016-01-21
Answer
You can see on the product datasheet, A00420-2 anti-MMP13 antibody has been validated for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse esophagus synovium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-21
Question
Do you have a BSA free version of anti-MMP13 antibody A00420-2 available?
C. Mangal
Verified customer
Asked: 2015-04-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MMP13 antibody A00420-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-04-10
Question
Our team were happy with the WB result of your anti-MMP13 antibody. However we have been able to see positive staining in mammary carcinoma extracellular space using this antibody. Is that expected? Could you tell me where is MMP13 supposed to be expressed?
S. Parker
Verified customer
Asked: 2013-04-05
Answer
From literature, mammary carcinoma does express MMP13. Generally MMP13 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP13 expression, here are a few articles citing expression in various tissues:
Esophagus, and Synovium, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Mammary carcinoma, Pubmed ID: 8207000, 9562863
Boster Scientific Support
Answered: 2013-04-05
