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Mmp13 antibody (A00420-2) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 32 Publications

A00420 2 MMP13 primary antibodies IHC testing 2

IHC analysis of MMP13 using anti-MMP13 antibody (A00420-2).
MMP13 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP13 Antibody (A00420-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Anti-MMP13 Antibody Picoband®

Mmp13 antibody

32 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, IHC, WB

Tested Applications

Mmp13 antibody was positively detected by WB in:

human A549 whole cell, human PC-3 whole cell, human U2OS whole cell, human HEK293 whole cell, rat testicular tissue, mouse testicular tissue,

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Mmp13 antibody was positively detected by IHC in:

mouse lung tissue, mouse small intestine tissue, rat lung tissue, rat small intestine tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-MMP13 Antibody Picoband®
View all MMP13 Antibodies

Form

Lyophilized

Uniprot

P23097

Gene ID

171052

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived rat MMP13 recombinant protein (Position: Y99-H335).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

54 kDa

Calculated Molecular Weight

53.4 kDa

Background of Mmp13

Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11.

Cite This Product

Anti-MMP13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00420-2)

Protocols

Western Blot protocol for Mmp13 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Mmp13 antibody

Review Protocol

Publications

CDs-PEI/siIhh Delivery System for the Treatment of Osteoarthritis

Published: 2026-05-26

Journal: ACS Applied Bio Materials

Application: WB

Impact Factor: 5.60

Species: Mouse

PMID: 42187059

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Anti-MMP13 Antibody Picoband®

Mmp13 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

CDs-PEI/siIhh Delivery System for the Treatment of Osteoarthritis

Structural alterations in osteocyte dendrites and lacunar-canalicular networks of the mandible of ovariectomized mice

Gluteal adipose-derived stem cell exosomes promote macrophage polarization and cartilage regeneration in osteoarthritis via PTPRC modulation

Spatiotemporally Controllable In Situ Hydrogels Enabled by Nanobottle-Mediated Encapsulation Technology for Stage-Specific Synergistic Therapy of Osteoarthritis

NsPEFs-enriched ADSCs-EVs alleviate osteoarthritis via RSPO3-mediated dual pro-chondrogenic and pro-M2 macrophage properties

Ipriflavone ameliorates intervertebral disc degeneration by inhibiting osteoporosis of vertebral body and pyroptosis of the nucleus pulposus in instability of lumbar spine and diabetic mice

Chondroprotective effects of ozone and hyaluronic acid in rat knee osteoarthritis: Comparison of intra-articular and systemic administration

Engineering natural arginine finger to deliver phosphate-containing drugs for treating osteoarthritis

Bioinspired exosome-SiO2 nanohybrid therapeutic for rheumatoid arthritis treatment

1,25(OH)2D3 induces chondrocyte autophagy and reduces the loss of proteoglycans in osteoarthritis through inhibiting the NF-κB pathway

Notopterol mitigates IL-1β-triggered pyroptosis by blocking NLRP3 inflammasome via the JAK2/NF-kB/hsa-miR-4282 route in osteoarthritis

High drug loading hydrophobic cross-linked dextran microspheres as novel drug delivery systems for the treatment of osteoarthritis

Pyruvate Dehydrogenase Kinase 1 inhibition mediated oxidative phosphorylation enhancement in cartilage promotes osteoarthritis progression

ADRM1/RPN13 attenuates cartilage extracellular matrix degradation via enhancing UCH37-mediated ALK5 deubiquitination

Pelargonidin ameliorates inflammatory response and cartilage degeneration in osteoarthritis via suppressing the NF-κB pathway

Identification of several inflammation-related genes based on bioinformatics and experiments

Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments

NIR-driven polydopamine-based nanoenzymes as ROS scavengers to suppress osteoarthritis progression

Dimethylamino group modified polydopamine nanoparticles with positive charges to scavenge cell-free DNA for rheumatoid arthritis therapy

Improving the symptoms of post-traumatic osteoarthritis by α2-macroglobulin-rich serum

Serum matrix metalloproteinase-13 as a diagnostic biomarker for cutaneous squamous cell carcinoma

Carbazate-modified cross-linked dextran microparticles suppress the progression of osteoarthritis by ROS scavenging

Dietary supplementation with Celecoxib to prevent the welfare problem of tibial dyschondroplasia in broiler chickens

Allogeneic adipose-derived mesenchymal stem cells promote the expression of chondrocyte redifferentiation markers and retard the progression of knee osteoarthritis in rabbits

Electroacupuncture Prevents Osteoarthritis of High-Fat Diet-Induced Obese Rats

Cartilage-targeting and dual MMP-13/pH responsive theranostic nanoprobes for osteoarthritis imaging and precision therapy

Dopamine-melanin nanoparticles scavenge reactive oxygen and nitrogen species and activate autophagy for osteoarthritis therapy

Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway

Involvement of S100A4/Mts1 and associated proteins in the protective effect of fluoxetine against MCT – Induced pulmonary hypertension in rats

Effects of ultrasound on estradiol level, bone mineral density, bone biomechanics and matrix metalloproteinase‑13 expression in ovariectomized rabbits

Enhancement of subchondral bone quality by alendronate administration for the reduction of cartilage degeneration in the early phase of experimental osteoarthritis

Effects of 17 β-estradiol on the expression of interstitial collagenases-8 and −13 (MMP-8 and MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in ovariectomized rat osteoblastic cells

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