Product Info Summary
SKU: | A11302 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Amphoterin-induced protein 1 AMIGO1 Antibody
SKU/Catalog Number
A11302
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Amphoterin-induced protein 1 AMIGO1 Antibody (Catalog # A11302). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
AMIGO1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Amphoterin-induced protein 1 AMIGO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11302)
Host
Rabbit
Contents
AMIGO1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
AMIGO1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AMIGO1. The immunogen is located within the last 50 amino acids of AMIGO1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
AMIGO1 antibody is human, mouse, and rat reactive. AMIGO1 antibody is predicted to not cross-react with AMIGO2 or AMIGO3.
Reactive Species
A11302 is reactive to AMIGO1 in Human, Mouse, Rat
Applications
A11302 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
55.239kDa
Background of AMIGO
The amphoterin-induced gene and ORF (AMIGO1) protein is a brain-enriched, glycosylated transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine-rich repeats (LRRs) and one Ig-like domain. It and the related proteins AMIGO2 and AMIGO3 are thought to be cell adhesion molecules expressed on fiber tracts of neuronal tissues and participate in their formation (1). AMIGO1 has also been suggested to play important roles in dendritic outgrowth during development and could modulate the survival of developing and adult neurons (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AMIGO1 antibody can be used for detection of AMIGO1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AMIGO1 in HeLa cell lysate with AMIGO1 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of AMIGO1 in human brain tissue with AMIGO1 antibody at 5 μg/mL.
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Immunofluorescence of AMIGO1 in human brain tissue with AMIGO1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For AMIGO1 (Source: Uniprot.org, NCBI)
Gene Name
AMIGO1
Full Name
Amphoterin-induced protein 1
Weight
55.239kDa
Superfamily
immunoglobulin superfamily
Alternative Names
adhesion molecule with Ig-like domain 1; ali2; Alivin-2; AMIGO; AMIGO1; AMIGO-1; AMIGOamphoterin-induced protein 1; amphoterin-induced gene and ORF; KIAA1163 AMIGO1 ALI2, AMIGO, AMIGO-1 adhesion molecule with Ig like domain 1 amphoterin-induced protein 1|alivin-2|amphoterin-induced gene and ORF
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AMIGO1, check out the AMIGO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AMIGO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Amphoterin-induced protein 1 AMIGO1 Antibody (A11302)
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