Data & Images
|Product Name||Anti-Olig1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Oligodendrocyte transcription factor 1(OLIG1) detection. Tested with WB in Human.|
|Cite This Product||Anti-Olig1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2213)|
|Replacement Item||This antibody may replace the following items: sc-83742|sc-19965|sc-83741|sc-48787|sc-83740|sc-374294|sc-166256|sc-166257|sc-166327|sc-373679 from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Olig1(94-109aa ARPDAKEEQQQQLRRK), different from the related mouse and rat sequences by four amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Oligodendrocyte transcription factor 1|
|Molecular Weight||27905 MW|
|Protein Function||Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube (By similarity). .|
|Tissue Specificity||Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable. .|
|Sequence Similarities||Contains 1 bHLH (basic helix-loop-helix) domain.|
|Subcellular Localization||Nucleus .|
|Alternative Names||Oligodendrocyte transcription factor 1;Oligo1;Class B basic helix-loop-helix protein 6;bHLHb6;Class E basic helix-loop-helix protein 21;bHLHe21;OLIG1;BHLHB6, BHLHE21;|
|Research Areas|||neuroscience|cell type marker|glia marker|oligodendrocyte marker| neuroscience|neurology process|neurogenesis||
Background for Oligodendrocyte transcription factor 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Olig1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Olig1 Antibody Images
Click the images to enlarge.
All lanes: Anti OLIG1 (PA2213) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,