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Product Info Summary
| SKU: | A02247-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-OLIG2 Antibody
SKU/Catalog Number
A02247-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-OLIG2 Antibody (Catalog # A02247-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
OLIG2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-OLIG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02247-1)
Host
Rabbit
Contents
OLIG2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
OLIG2 antibody was raised against a 15 amino acid peptide near the amino terminus of human OLIG2. The immunogen is located within amino acids 80 - 130 of OLIG2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
OLIG2 antibody is human, mouse and rat reactive. It is predicted to not cross-react with other members of the OLIG family of proteins. At least three isoforms of OLIG2 are known to exist; this antibody will detect all three isoforms.
Reactive Species
A02247-1 is reactive to OLIG2 in Human, Mouse, Rat
Applications
A02247-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
32.385kDa
Background of OLIG2
The oligodendrocyte transcription factors 1 and 2 (OLIG1 and OLIG2, respectively) make up part of basic helix-loop-helix (bHLH) family of transcription factors that are specifically expressed in zones of the neuroepithelium from which oligodendrocyte precursors emerge (1). Both OLIG1 and OLIG2 genes are downstream targets of Sonic hedgehog and are expressed exclusively in the central nervous system (2). OLIG2 is first observed in the ventral most p3 progenitor domain of the ventral neural tube while OLIG1 is first expressed in the dorsal portion of the p3 domain (2). Mice overexpressing OLIG2 exhibit impaired potassium channel expression in neural progenitors and proliferation of these cells similar to that seen in Down Syndrome, suggesting that OLIG2 may play a role in this pathology (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
OLIG2 antibody can be used for detection of OLIG2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of OLIG2 in EL4 cell lysate with OLIG2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of OLIG2 in rat brain tissue with OLIG2 antibody at 5 μg/mL.
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Immunofluorescence of OLIG2 in rat brain tissue with OLIG2 antibody at 20 μg/mL.
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Immunohistochemistry of OLIG2 in mouse brain tissue with OLIG2 Antibody at 5 μg/mL.
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Immunofluorescence of OLIG2 in mouse brain tissue with OLIG2 Antibody at 20 μg/mL.
Green: OLIG2 antibody (A02247-1)
Red: Phylloidin staining
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For OLIG2 (Source: Uniprot.org, NCBI)
Gene Name
OLIG2
Full Name
Oligodendrocyte transcription factor 2
Weight
32.385kDa
Alternative Names
BHLHB1; BHLHE19; Class B basic helix-loop-helix protein 1; Class E basic helix-loop-helix protein 19; human protein kinase C-binding protein RACK17; Olig2; OLIGO2; oligodendrocyte lineage transcription factor 2; oligodendrocyte transcription factor 2; oligodendrocyte-specific bHLH transcription factor 2; PRKCBP2; protein kinase C binding protein 2; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; RACK17; RACK17helix-loop-helix protein, class B, 1 OLIG2 BHLHB1, OLIGO2, PRKCBP2, RACK17, bHLHe19 oligodendrocyte transcription factor 2 oligodendrocyte transcription factor 2|basic domain, helix-loop-helix protein, class B, 1|class B basic helix-loop-helix protein 1|class E basic helix-loop-helix protein 19|human protein kinase C-binding protein RACK17|oligodendrocyte lineage transcription factor 2|oligodendrocyte-specific bHLH transcription factor 2|protein kinase C-binding protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OLIG2, check out the OLIG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OLIG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OLIG2 Antibody (A02247-1)
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