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Product Info Summary
| SKU: | A02758-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-AMPK gamma2 PRKAG2 Antibody
SKU/Catalog Number
A02758-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-AMPK gamma2 PRKAG2 Antibody catalog # A02758-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMPK gamma2 PRKAG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02758-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
8C4C4
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PRKAG2. AA range:1-50
Reactive Species
A02758-1 is reactive to PRKAG2 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
63.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02758-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of K562 using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using PRKAG2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549 cells, using PRKAG2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-AMPK gamma2 PRKAG2 Antibody (A02758-1)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-AMPK gamma2 PRKAG2 Antibody
Question
Is a blocking peptide available for product anti-AMPK gamma2 antibody (A02758-1)?
G. Johnson
Verified customer
Asked: 2020-05-04
Answer
We do provide the blocking peptide for product anti-AMPK gamma2 antibody (A02758-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-05-04
Question
Do you have a BSA free version of anti-AMPK gamma2 antibody A02758-1 available?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-AMPK gamma2 antibody A02758-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-04
Question
We want to test anti-AMPK gamma2 antibody A02758-1 on human liver for research purposes, then I may be interested in using anti-AMPK gamma2 antibody A02758-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-18
Answer
The products we sell, including anti-AMPK gamma2 antibody A02758-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-18
Question
Would anti-AMPK gamma2 antibody A02758-1 work for WB with liver?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
According to the expression profile of liver, PRKAG2 is highly expressed in liver. So, it is likely that anti-AMPK gamma2 antibody A02758-1 will work for WB with liver.
Boster Scientific Support
Answered: 2017-07-05
Question
We are currently using anti-AMPK gamma2 antibody A02758-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
The anti-AMPK gamma2 antibody (A02758-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-AMPK gamma2 antibody A02758-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-19
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-19
