Anti-LAMP-2 Antibody

Product Name

Anti-LAMP-2 Antibody

View all LAMP2 Antibodies

SKU/Catalog Number

A01573

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LAMP-2 Antibody (Catalog # A01573). Tested in ELISA, WB, ICC, IHC-P applications. This antibody reacts with Human, Mouse.

Storage & Handling

LAMP-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LAMP-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01573)

Host

Rabbit

Contents

LAMP-2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LAMP-2 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human LAMP-2. The immunogen is located within the last 50 amino acids of LAMP-2.

Cross-reactivity

LAMP-2 antibody cross reacts with LAMP2B and LAMP2C, but not LAMP2A.

Reactive Species

A01573 is reactive to LAMP2 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

45.7 kDa

Background of LAMP2

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). LAMP-2, a highly glycosylated protein associated with the lysosome, has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes. LAMP-2 exists in multiple isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01573 is guaranteed for ELISA, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LAMP-2 antibody can be used for the detection of LAMP-2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information LAMP2

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