Anti-Angiopoietin-related protein 1 ANGPTL1 Antibody Picoband®

Product Name

Anti-Angiopoietin-related protein 1 ANGPTL1 Antibody Picoband®

View all ANGPTL1 Antibodies

SKU/Catalog Number

PA1487
BA3582-2 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

ANGPTL1 (angiopoietin-like 1) is a secreted glycoprotein implicated in vascular biology and angiogenic regulation, with frequent use in oncology/vascular inflammation research as an angiogenesis-modulating factor (putative where context-specific). Assay context: primary antibody validated for Western blot with Human/Mouse/Rat reactivity; ANGPTL1 signaling/phenotypes are often interpreted alongside membrane-trafficking and integrin-axis regulators such as ARF6 (putative mechanistic adjacency) and efferocytosis/repair mediators like MFGE8 (putative)."

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Angiopoietin-related protein 1 ANGPTL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1487)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ANGPTL1, identical to the related rat and mouse sequences.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1487 is reactive to ANGPTL1 in Human, Mouse, Rat

Observed Molecular Weight

55-58 kDa

Calculated molecular weight

56.7 kDa

Background of ANGPTL1

ANGPTL1, Angiopoietin-related protein 1, is a protein that in humans is encoded by the ANGPTL1 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. The protein encoded by this gene is another member of the angiopoietin family that is widely expressed in adult tissues with mRNA levels highest in highly vascularized tissues. This protein was found to be a secretory protein that does not act as an endothelial cell mitogen in vitro. ANGPTL1 is widely expressed in human adult tissues as a major 3.0-kb transcript and a less abundant 4.0-kb transcript.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1487 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: A549 Cell, SW620 Cell, MCF-7 Cell, MM231 Cell

Validation Images & Assay Conditions

See full target information ANGPTL1

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