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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human FGFBP1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human FGFBP1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FGFBP1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FGFBP1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1706)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: K24-C234
23.4 pg/ml - 1500 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1706 is reactive to FGFBP1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1706 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FGF-BP
Fibroblast growth factor-binding protein 1 is a protein that in humans is encoded by the FGFBP1 gene. This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 4.
|EK1706-CAP||Anti-Human FGFBP1 Pre-coated 96-well strip microplate||1|
|EK1706-ST||Human FGFBP1 Standard||2 vials, 10 ng/tube|
|EK1706-DA||Human FGFBP1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human FGFBP1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FGF-BP, Dilution ratio of 1:10, concentration in serum is around 1.7ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FGFBP1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of FGF-BP in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
|Sample . 2||158||181||166||164||167||8.46||5.0%%|
Gene/Protein Information For FGFBP1 (Source: Uniprot.Org, NCBI)
Fibroblast growth factor-binding protein 1
fibroblast growth factor-binding protein family
17 kDa heparin-binding growth factor-binding protein; FGFBP; FGF-BP; FGFBP1; FGF-BP1; FGFBP-1; FGFBPFGF-binding protein 1; fibroblast growth factor binding protein 1; fibroblast growth factor-binding protein 1; HBp17; HBP1717 kDa HBGF-binding protein; heparin-binding growth factor binding protein FGFBP1 FGF-BP, FGF-BP1, FGFBP, FGFBP-1, HBP17 fibroblast growth factor binding protein 1 fibroblast growth factor-binding protein 1|17 kDa HBGF-binding protein|17 kDa heparin-binding growth factor-binding protein|FGF-binding protein 1|heparin-binding growth factor binding protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FGFBP1, check out the FGFBP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FGFBP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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