Click image to see more details
Product Info Summary
| SKU: | M02929 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ANGPTL3 Mouse Monoclonal Antibody [Clone ID: OTI5E6]
SKU/Catalog Number
M02929
Size
100 µl
Description
Boster Bio ANGPTL3 mouse monoclonal antibody, clone OTI5E6 (formerly 5E6). Catalog# M02929. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ANGPTL3 Mouse Monoclonal Antibody [Clone ID: OTI5E6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02929)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI5E6
Isotype
IgG2b
Immunogen
Human recombinant protein fragment corresponding to amino acids 237-460 of human ANGPTL3 (NP_055310) produced in E.coli.
Reactive Species
M02929 is reactive to ANGPTL3 in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
53.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02929 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:150
Positive Control
WB: human HepG2 whole cell, human A375 whole cell, rat liver tissue, mouse liver tissue
IHC: Human pancreas tissue, Human colon tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ANGPTL3 using anti-ANGPTL3 antibody (M02929).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ANGPTL3 antigen affinity purified monoclonal antibody (M02929) at 1: 2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ANGPTL3 at approximately 54 kDa. The expected band size for ANGPTL3 is at 54 kDa.
Click image to see more details
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ANGPTL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Click image to see more details
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ANGPTL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Specific Publications For Anti-ANGPTL3 Mouse Monoclonal Antibody [Clone ID: OTI5E6] (M02929)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ANGPTL3 Mouse Monoclonal Antibody [Clone ID: OTI5E6]?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-ANGPTL3 Mouse Monoclonal Antibody [Clone ID: OTI5E6]
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
