Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine™

Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PCSK9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK1147
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine™

See all Proprotein Convertase 9/PCSK9 products

SKU/Catalog Number

EK1147

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PCSK9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1147)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S153-Q692

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1147 is reactive to PCSK9 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1147 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1147-CAP Anti-Human PCSK9 Pre-coated 96-well strip microplate 1
EK1147-ST Human PCSK9 Standard 2 vials, 10 ng/tube
EK1147-DA Human PCSK9 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0130.0620.1020.1690.3560.7431.3272.221

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Proprotein Convertase 9/PCSK9, Dilution ratio of 1:50, concentration in serum is around 10ng/ml.

Some articles we found to cite concentrations of Proprotein Convertase 9/PCSK9 in samples: 19571328 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2291991540421519275944
Standard deviation16.03125.43243.1817.63132.96285.31
CV (%)7%6.9%4.5%8.2%6.9%4.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Proprotein Convertase 9/PCSK9 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 122922921919121715.557.1%
Sample 219911900165518871858123.996.6%
Sample 354044654467448654899302.836.1%
*number of samples for each test n=16.

Gene/Protein Information For PCSK9 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8NBP7

Gene ID

255738

Gene Name

PCSK9

Full Name

Proprotein convertase subtilisin/kexin type 9

Weight

74.286kDa

Superfamily

peptidase S8 family

Alternative Names

EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; FHCL3; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9 PCSK9 FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9 proprotein convertase subtilisin/kexin type 9 proprotein convertase subtilisin/kexin type 9|convertase subtilisin/kexin type 9 preproprotein|neural apoptosis regulated convertase 1|subtilisin/kexin-like protease PC9

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PCSK9, check out the PCSK9 Infographic

PCSK9 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PCSK9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1147 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

RGC-32 (Response Gene to Complement 32) Deficiency Protects Endothelial Cells From Inflammation and Attenuates Atherosclerosis

Thyroid stimulating hormone exhibits the impact on LDLR/LDL-c via up-regulating hepatic PCSK9 expression

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9 Customer Q&As for Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated PCSK9 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PCSK9 antigen?

L. Hill

Verified customer

Asked: 2020-11-28

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-11-28

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

W. Maharaj

Verified customer

Asked: 2020-09-12

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-09-12

Question

Q: What is the optimal O.D. value for PCSK9 ELISA kit? I performed your PCSK9 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain PCSK9 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-06-10

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PCSK9 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of PCSK9 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-06-10

Question

Q: how do I analyze ELISA data? I have performed PCSK9 level in cell lysates.

R. Perez

Verified customer

Asked: 2020-04-13

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-04-13

Question

Can your human PCSK9 ELISA Kit detect distinguishably the difference of heterodimer and free fragment of human PCSK9? Keyword: samples, form, application

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

Our human PCSK9 ELISA Kit (EK1147) detects both heterodimer and free fragment of human PCSK9. It cannot detect the difference between them.

Boster Scientific Support

Answered: 2020-02-21

Question

Does the Human PCSK9/Proprotein Convertase 9 PicoKine

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

Human PCSK9/Proprotein Convertase 9 PicoKine

Boster Scientific Support

Answered: 2020-02-18

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-05-01

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-05-01

Question

Q: Can PCSK9 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

W. Williams

Verified customer

Asked: 2018-07-21

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-07-21

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement PCSK9 using the EK1147 Human PCSK9 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-07-13

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2018-07-13

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