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- Table of Contents
ANXA10 antibody (A13560-1) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of PC-3 cells using anti-Annexin A10/ANXA10 antibody (A13560-1).
Overlay histogram showing PC-3 cells stained with A13560-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin A10/ANXA10 Antibody (A13560-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing PC-3 cells stained with A13560-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin A10/ANXA10 Antibody (A13560-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Annexin A10/ANXA10 Antibody Picoband®
ANXA10 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
ANXA10 antibody was positively detected by WB in:
human CACO-2 whole cell, human Hela whole cell, human A431 whole cell, human K562 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ANXA10 antibody was positively detected by IHC in:
human appendiceal adenocarcinoma tissue, human thyroid cancer tissue, human lung adenocarcinoma tissue, human placenta tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ANXA10 antibody was positively detected by IF in:
human placenta tissue
ANXA10 antibody was positively detected by FC in:
PC-3 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml/ml
Human
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/ml/1x10^6 cells
Human
ELISA
0.1-0.5 μg/ml/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Annexin A10/ANXA10 Antibody Picoband®
View all ANXA10 Antibodies
View all ANXA10 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Annexin A10/ANXA10 recombinant protein (Position: N49-Y324).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
37.3 kDa
Background of ANXA10
ANXA10(Annexin A10) is a member of the annexin family of calcium-dependent phospholipid-binding proteins. By FISH, Morgan et al.(1999) mapped the ANXA10 gene to chromosome 4q33. Morgan et al.(1999) suggested that the ablation or depletion of principal calcium-binding sites in annexins A9 and A10, respectively, may dispose them to function in distinct tissue environments where calcium sensitivity is not a major functional determinant.
Cite This Product
Anti-Annexin A10/ANXA10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13560-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
93%
High Homology
Mouse
89%
Moderate Homology
Rat
89%
Moderate Homology
Cow
86%
Moderate Homology
Sheep
84%
Moderate Homology
Zebrafish
44%
Low homology
Drosophila
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ANXA10 antibody
Immunohistochemistry (IHC) protocol for ANXA10 antibody
Immunofluorescence (IF) protocol for ANXA10 antibody
Flow Cytometry (FC) protocol for ANXA10 antibody
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Product Promise
We promise Anti-Annexin A10/ANXA10 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A13560-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026