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Product Info Summary
| SKU: | A01315-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TCF-1 (F26) TCF7 Antibody
SKU/Catalog Number
A01315-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TCF-1 (F26) TCF7 Antibody catalog # A01315-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCF-1 (F26) TCF7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01315-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids N-terminus of Human TCF-1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01315-1 is reactive to TCF7 in Human, Mouse, Rat
Calculated molecular weight
41.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01315-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of TCF-1 (F26) pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:MEF whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of TCF-1 (F26) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Immunofluorescence analysis of L02 cells using TCF-1 (F26) pAb at dilution of 1:200 (40x lens).
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Immunoprecipitation of HCT116 cell lysates using TCF-1 pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using TCF-1 pAb.
Specific Publications For Anti-TCF-1 (F26) TCF7 Antibody (A01315-1)
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