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Product Info Summary
| SKU: | M04796 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Annexin A3 (ANXA3) Mouse Monoclonal Antibody [Clone ID: OTI1A5]
SKU/Catalog Number
M04796
Size
100 µl
Description
Boster Bio ANXA3 (Annexin A3) mouse monoclonal antibody, clone OTI1A5 (formerly 1A5). Catalog# M04796. Tested in FC, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Annexin A3 (ANXA3) Mouse Monoclonal Antibody [Clone ID: OTI1A5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04796)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1A5
Isotype
IgG1
Immunogen
Full length human recombinant protein of human ANXA3 (NP_005130) produced in HEK293T cell.
Reactive Species
M04796 is reactive to ANXA3 in Human, Mouse, Rat
Calculated molecular weight
36.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04796 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
IHC 1:150
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA3.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANXA3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ANXA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ANXA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ANXA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either ANXA3 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANXA3 antibody (M04796)
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and ANXA3-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ANXA3 monoclonal antibody M04796 (1:200`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Specific Publications For Anti-Annexin A3 (ANXA3) Mouse Monoclonal Antibody [Clone ID: OTI1A5] (M04796)
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