Anti-Annexin A3/ANXA3 Antibody Picoband™

Annexin A3 antibody

Boster Bio Anti-Annexin A3/ANXA3 Antibody Picoband™ catalog # A04796. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A04796
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Annexin A3/ANXA3 Antibody Picoband™

View all Annexin A3 Antibodies

SKU/Catalog Number

A04796

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Annexin A3/ANXA3 Antibody Picoband™ catalog # A04796. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Annexin A3/ANXA3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04796)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Annexin A3/ANXA3 recombinant protein (Position: D23-D323).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A04796 is reactive to ANXA3 in Human, Mouse, Rat

Applications

A04796 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of Annexin A3

Annexin A3 is a protein that in humans is encoded by the Annexin A3 gene. The Annexin A3 gene contains 13 exons and spans 58 kb of genomic DNA. The Annexin A3 gene is mapped to 4q21. It is abnormally expressed in fetuses of both IVF and ICSI, which may contribute to the increase risk of birth defects in these ART. This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phospholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For ANXA3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P12429

Gene ID

306

Gene Name

ANXA3

Full Name

Annexin A3

Weight

36.375kDa

Superfamily

annexin family

Alternative Names

35-alpha calcimedin; Annexin A3; annexin A3,1,2-cyclic-inositol-phosphate phosphodiesterase; Annexin III; annexin-3; ANXA3; Calcimedin 35-alpha ; calcimedin 35-alpha; Lipocortin III; PAP-III; Placental anticoagulant protein III; placental anticoagulant protein III, calcimedin 35-alpha ANXA3 ANX3 annexin A3 annexin A3|35-alpha calcimedin|Annexin III (lipocortin III)|PAP-III|annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha)|annexin-3|calcimedin 35-alpha|inositol 1,2-cyclic phosphate 2-phosphohydrolase|lipocortin III|placental anticoagulant protein III

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANXA3, check out the ANXA3 Infographic

ANXA3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANXA3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A04796 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection

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12 Customer Q&As for Anti-Annexin A3/ANXA3 Antibody Picoband™

Question

Is a blocking peptide available for product anti-Annexin A3/ANXA3 antibody (A04796)?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

We do provide the blocking peptide for product anti-Annexin A3/ANXA3 antibody (A04796). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-24

Question

I was wanting to use your anti-Annexin A3/ANXA3 antibody for WB for rat bone marrow on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat bone marrow identification?

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

You can see on the product datasheet, A04796 anti-Annexin A3/ANXA3 antibody has been validated for ELISA, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat bone marrow in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-24

Question

Do you have a BSA free version of anti-Annexin A3/ANXA3 antibody A04796 available?

C. Krishna

Verified customer

Asked: 2019-12-24

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Annexin A3/ANXA3 antibody A04796 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-24

Question

Can you help my question with product A04796, anti-Annexin A3/ANXA3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04796 anti-Annexin A3/ANXA3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-25

Question

Will A04796 anti-Annexin A3/ANXA3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Rodriguez

Verified customer

Asked: 2019-09-10

Answer

As indicated on the product datasheet, A04796 anti-Annexin A3/ANXA3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-10

Question

Would anti-Annexin A3/ANXA3 antibody A04796 work on horse IHC-P with platelet?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

Our lab technicians have not validated anti-Annexin A3/ANXA3 antibody A04796 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Annexin A3/ANXA3 antibody A04796 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse platelet in IHC-P, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-29

Question

Will anti-Annexin A3/ANXA3 antibody A04796 work for WB with bone marrow?

D. Li

Verified customer

Asked: 2019-08-19

Answer

According to the expression profile of bone marrow, ANXA3 is highly expressed in bone marrow. So, it is likely that anti-Annexin A3/ANXA3 antibody A04796 will work for WB with bone marrow.

Boster Scientific Support

Answered: 2019-08-19

Question

I was wanting to use to test anti-Annexin A3/ANXA3 antibody A04796 on rat bone marrow for research purposes, then I may be interested in using anti-Annexin A3/ANXA3 antibody A04796 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-12-19

Answer

The products we sell, including anti-Annexin A3/ANXA3 antibody A04796, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-12-19

Question

I have attached the WB image, lot number and protocol we used for bone marrow using anti-Annexin A3/ANXA3 antibody A04796. Please let me know if you require anything else.

C. Taylor

Verified customer

Asked: 2016-08-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-08-17

Question

Is this A04796 anti-Annexin A3/ANXA3 antibody reactive to the isotypes of ANXA3?

H. Yang

Verified customer

Asked: 2014-10-15

Answer

The immunogen of A04796 anti-Annexin A3/ANXA3 antibody is E.coli-derived human Annexin A3/ANXA3 recombinant protein (Position: D23-D323). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-10-15

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-Annexin A3/ANXA3 antibody A04796. Let me know if you need anything else.

E. Taylor

Verified customer

Asked: 2013-07-16

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-07-16

Question

I see that the anti-Annexin A3/ANXA3 antibody A04796 works with WB, what is the protocol used to produce the result images on the product page?

S. Zhang

Verified customer

Asked: 2013-01-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-01-23

Conjugation

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Size

Total: $315

Size:100μg

SKU:A04796

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A04796
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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