Click image to see more details
Product Info Summary
| SKU: | P03471-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-APC1 phospho S355 mat-2 Antibody
View all ANAPC1, TSG24 Antibodies
SKU/Catalog Number
P03471-2
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-APC1 phospho S355 mat-2 Antibody (Catalog # P03471-2). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-APC1 phospho S355 mat-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03471-2)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human Apc1 protein.
Reactive Species
P03471-2 is reactive to ANAPC1, TSG24 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
216.5 kDa
Background of ANAPC1, TSG24
APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03471-2 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:35,000
IP: 1:100
WB: 1:200 - 1:2,000
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 215 kDa in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.
Validation Images & Assay Conditions
Click image to see more details
Western blot using Boster's Affinity Purified anti-APC1 pS355 antibody shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary. Lane 3 shows lysate from cells treated with nocodazole to synchronize cells at the M phase. Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division. Each lane contains approximately 30 µg of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose. After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1:500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX (611-103-122) for 45 min at room temperature. ECL reagent was used for detection. Other detection systems will yield similar results. Data contributed by Bing Li, UT Southwestern.
Specific Publications For Anti-APC1 phospho S355 mat-2 Antibody (P03471-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-APC1 phospho S355 mat-2 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-APC1 phospho S355 mat-2 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
