Human IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Product Name

Human IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all IL12A products

SKU/Catalog Number

EZ0421

Size

For 5 plates, 96 wells each, reagents only, plates are not included

*Question: How many samples can I assay/run in this kit?

Description

IL-12 (p70) is the IL12A(p35)–IL12B(p40) heterodimer that promotes Th1 differentiation and links innate-to-adaptive immunity (IFN-γ–biased responses; STAT4 signaling described in curated protein resources). Assay context: EZ-Set™ DIY antibody-pair format for developing a sandwich ELISA measuring Human IL-12(p70) in culture supernatants and serum; datasheet notes a 7.8–500 pg/mL range, capture (mouse mAb) + biotin detection (goat pAb), and plate incubation options. Often profiled alongside immune-checkpoint ligand PD-L1/CD274 in immuno-oncology or inflammatory studies, and can be extended to multi-cytokine readouts using multiplex assays (putative).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0421)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: (I23-S328) + (R23-S219)

Assay Range

7.8 pg/ml - 500 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0421 is reactive to IL12A in Human samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EZ0421 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number	Description	Quantity	Storage of opened/reconstituted material
EZ0421-CA	Mouse anti- human IL-12(p70) monoclonal antibody (Capture Antibody)	500 µl, 0.4 mg/mL (recommended dilution 1:100)	Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0421-DA	Biotinylated goat anti- human IL-12(p70) polyclonal antibody (Detection Antibody)	500 µl, 7.2 µg/mL (recommended dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	500 µl (recommended dilution 1:100)
EK0421-ST	Lyophilized recombinant human IL-12(p70) standard	10 ng/tube×3	Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.019	0.069	0.126	0.239	0.441	0.834	1.441	2.086

Data Example Images

Gene/Protein Information For IL12A (Source: Uniprot.Org, NCBI)

Alternative Names

Interleukin 12 ;Interleukin 12, P35 ;Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) ;Interleukin 12A (Natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35), isoform CRA_a ;IL12A ;hCG_1685939 ;

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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