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Product Info Summary
|Size:||For 5 plates, 96 wells each, reagents only, plates are not included|
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Human IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs)
For 5 plates, 96 wells each, reagents only, plates are not included
*Question: How many samples can I assay/run in this kit?
Human IL-12 (p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL-12 (p70) in cell culture supernatants and serum. Sensitivity: 2 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0421)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: (I23-S328) + (R23-S219)
7.8 pg/ml - 500 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EZ0421 is reactive to IL-12 (p70) in Human samples
Validated Sample Types
cell culture supernatants and serum.
EZ0421 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|Catalog number||Description||Quantity||Storage of opened/reconstituted material|
|EZ0421-CA||Mouse anti- human IL-12 (p70) monoclonal antibody (Capture Antibody)||500 µl, 0.4 mg/mL (recommended dilution 1:100)||Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.|
|EZ0421-DA||Biotinylated goat anti- human IL-12 (p70) polyclonal antibody (Detection Antibody)||500 µl, 7.2 µg/mL (recommended dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex (ABC)||500 µl (recommended dilution 1:100)|
|EZ0421-ST||Lyophilized recombinant human IL-12 (p70) standard||10 ng/tube×3||Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. 96 well microplate (Cat# AR1100)
- 6. Plate Sealers.
- 7. Capture Antibody Diluent: PBS.
- 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 11. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human IL-12(p70) EZ Set ELISA Kit standard curve
Gene/Protein Information For IL-12 (p70) (Source: Uniprot.Org, NCBI)
Interleukin-12 subunit alpha
CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35 IL12A CLMF, IL-12A, NFSK, NKSF1, P35 interleukin 12A interleukin-12 subunit alpha|CLMF p35|IL-12, subunit p35|IL35 subunit|NF cell stimulatory factor chain 1|NK cell stimulatory factor chain 1|cytotoxic lymphocyte maturation factor 1, p35|cytotoxic lymphocyte maturation factor 35 kDa subunit|interleukin 12, p35|interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)|interleukin-12 alpha chain*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IL-12 (p70), check out the IL-12 (p70) Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL-12 (p70): database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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