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- Table of Contents
Il12a antibody (A00918-1) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IHC analysis of IL12A using anti-IL12A antibody (A00918-1).
IL12A was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL12A Antibody (A00918-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IL12A was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL12A Antibody (A00918-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-IL12A Antibody Picoband®
Il12a antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
Il12a antibody was positively detected by WB in:
human Hela cell, human SW620 cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Il12a antibody was positively detected by IHC in:
mouse small intestine tissue, mouse spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Recommend
Mouse
Tested
Recommend
Recommend
Rat
Recommend
Product Information
Product Name
Anti-IL12A Antibody Picoband®
View all IL12A Antibodies
View all IL12A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat IL12A recombinant protein (Position: R23-S215).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated Molecular Weight
24.2 kDa
Background of Il12a
Interleukin-12 subunit alpha (IL-12 p35) is a protein that in humans is encoded by the IL12A gene. This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
Cite This Product
Anti-IL12A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00918-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
87%
Moderate Homology
Rabbit
61%
Low homology
Human
60%
Low homology
Cow
60%
Low homology
Sheep
59%
Low homology
Monkey
59%
Low homology
Dog
58%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Il12a antibody
Immunohistochemistry (IHC) protocol for Il12a antibody
Publications
Application:
Species:
Supramolecular lipid nanoparticle reprograms tumor microenvironment by cucurbit[7]uril-based host–guest recognition for STING-activating cancer immunotherapy
Published: 2025-12-03
Journal: Materials Today
Application: IF
Impact Factor: 22.00
Species: Mouse
Evaluation of the Effectiveness of Active Vitamin D Use in Experimental Rat Lymphedema Model
Published: 2024-11-01
Journal: Medicina-Lithuania
Application: IHC
Impact Factor: 2.40
Species: Rat
PMID: 39596973
Exogenous IL-25 ameliorates airway neutrophilia via suppressing macrophage M1 polarization and the expression of IL-12 and IL-23 in asthma
Published: 2023-10-28
Journal: RESPIRATORY RESEARCH
Application: WB
Impact Factor: 5.80
Species: Mouse
PMID: 37898756
Interleukin-12 and interferon-γ acting on damp-heat of spleen-stomach syndrome triggered by helicobacter pylori
Published: 2018-02-08
Journal: Journal of Traditional Chinese Medicine
Application: IHC
Impact Factor: 0.86
Species: Human
Targeted depletion of tumour-associated macrophages by an alendronate–glucomannan conjugate for cancer immunotherapy
Published: 2014-09-22
Journal: BIOMATERIALS
Application: IF
Impact Factor: 8.31
Species: Mouse
PMID: 25245263
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Product Promise
We promise Anti-IL12A Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00918-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026