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ATG5 antibody (PA2260) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 14 Publications
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Flow Cytometry analysis of HepG2 cells using anti-ATG5 antibody (PA2260).
Overlay histogram showing CACO-2 cells stained with PA2260 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG5 Antibody (PA2260, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing CACO-2 cells stained with PA2260 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG5 Antibody (PA2260, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot or IHC for ATG5? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-APG5L/ATG5 Antibody Picoband®
ATG5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ATG5 antibody was positively detected by WB in:
human A549 whole cell, human Hela whole cell, human HepG2 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ATG5 antibody was positively detected by IHC in:
human ovarian cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ATG5 antibody was positively detected by ICC/IF in:
A549 cell
ATG5 antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5ug/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-APG5L/ATG5 Antibody Picoband®
View all ATG5 Antibodies
View all ATG5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human APG5L, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50-52 kDa
Calculated Molecular Weight
32.4 kDa
Background of ATG5
Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.
Cite This Product
Anti-APG5L/ATG5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2260)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Rat
94%
High Homology
Zebrafish
88%
Moderate Homology
Drosophila
69%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ATG5 antibody
Immunohistochemistry (IHC) protocol for ATG5 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ATG5 antibody
Flow Cytometry (FC) protocol for ATG5 antibody
Publications
Application:
Species:
mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via Piezo1-NCOA4-ZBP1 axis
Published: 2026-05-20
Journal: Autophagy
Application: WB
Impact Factor: 18.60
Species: Mouse
PMID: 42153663
rs2253820 Variant Controls Blood Pressure Dip After Stroke by Increasing CLOCK–BMAL1 Expression
Published: 2022-07-23
Journal: Translational Stroke Research
Application: WB
Impact Factor: 6.80
Species: Rat
PMID: 35870088
Iron-overloaded follicular fluid increases the risk of endometriosis-related infertility by triggering granulosa cell ferroptosis and oocyte dysmaturity
Published: 2022-07-04
Journal: Cell Death & Disease
Application: WB
Impact Factor: 9.69
Species: Mouse
PMID: 35787614
BCAT1 binds the RNA-binding protein ZNF423 to activate autophagy via the IRE1-XBP-1-RIDD axis in hypoxic PASMCs
Published: 2020-09-16
Journal: Cell Death & Disease
Application: IF,WB
Impact Factor: 6.30
Species: Rat
PMID: 32938905
Exogenous Hydrogen Sulfide Ameliorates Diabetic Myocardial Fibrosis by Inhibiting Cell Aging Through SIRT6/AMPK Autophagy
Published: 2020-07-31
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.23
Species: Rat
PMID: 32903815
ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro
Published: 2019-07-05
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY
Application: WB
Impact Factor: 3.59
Species: Human
PMID: 31283929
miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis
Published: 2019-04-07
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 3.45
Species: Human
PMID: 30970265
The effect of honokiol on pulmonary artery endothelium cell autophagy mediated by cyclophilin A in hypoxic pulmonary arterial hypertension
Published: 2019-02-01
Journal: JOURNAL OF PHARMACOLOGICAL SCIENCES
Application: WB
Impact Factor: 2.44
Species: Rat
PMID: 30770282
Andrographolide Induces Autophagic Cell Death and Inhibits Invasion and Metastasis of Human Osteosarcoma Cells in An Autophagy-Dependent Manner
Published: 2017-11-30
Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 5.10
Species: Human
PMID: 29197865
Endocan silencing induces programmed cell death in hepatocarcinoma
Published: 2017-08-31
Journal: Oncology Letters
Application: WB
Impact Factor: 1.39
Species: Human
PMID: 29113167
Association between genetic polymorphisms in the autophagy-related 5 gene promoter and the risk of sepsis
Published: 2017-08-24
Journal: Scientific Reports
Application: WB
Impact Factor: 4.26
Species: Human
PMID: 28839236
Antagomirs Targeting MiroRNA-134 Attenuates Epilepsy in Rats through Regulation of Oxidative Stress, Mitochondrial Functions and Autophagy
Published: 2017-08-08
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.40
Species: Rat
PMID: 28848439
Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1α and autophagy in human osteosarcoma
Published: 2017-08-03
Journal: Oncotarget
Application: WB
Impact Factor: 5.17
Species: Human
PMID: 29207631
Anti-autophagic and anti-apoptotic effects of memantine in a SH-SY5Y cell model of Alzheimer's disease via mammalian target of rapamycin-dependent and -independent pathways
Published: 2015-09-29
Journal: Molecular Medicine Reports
Impact Factor: 1.55
PMID: 26459718
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Product Promise
We promise Anti-APG5L/ATG5 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2260
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026