|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-APG5L/ATG5 Antibody|
|Description||Rabbit IgG polyclonal antibody for Autophagy protein 5(ATG5) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-APG5L/ATG5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2260)|
|Replacement Item||This antibody may replace the following items: sc-80537|sc-53514|sc-10174|sc-10176|sc-18856|sc-18855|sc-10169|sc-13535|sc-20929|sc-365423|sc-365506|sc-10172|sc-71940|sc-32302|sc-398402 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human APG5L(82-97aa DRFDQFWAINRKLMEY), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Autophagy protein 5|
|Molecular Weight||32447 MW|
|Protein Function||Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12- ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.|
|Tissue Specificity||Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.|
|Subcellular Localization||Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. .|
|Alternative Names||Autophagy protein 5;APG5-like;Apoptosis-specific protein;ATG5;APG5L, ASP;|
|Research Areas|||cell biology|apoptosis|intracellular|associated proteins| cell biology|proteolysis / ubiquitin|proteasome / ubiquitin|ub-like proteins|phagocytosis| cardiovascular|heart|autophagy|apg gene products| cancer|signal transduction| metabolism|pathways and processes|metabolism processes|autophagy and mitophagy|cell death||
Background for Autophagy protein 5
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-APG5L/ATG5 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-APG5L/ATG5 Antibody Images
Click the images to enlarge.
All lanes: Anti- ATG5(PA2260) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 4oug
Lane 2: Rat Spleen Tissue Lysate at 4oug
Lane 3: Rat Kidney Tissue Lysate at 4oug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Lane 6: NIH Whole Cell Lysate at 40ug
Lane 7: HEPG2 Whole Cell Lysate at 40ug
Lane 8: PC12 Whole Cell Lysate at 40ug
Lane 9: NRK Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 32KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,